Association Between Single Nucleotide Polymorphisms in miRNA196a-2 and miRNA146a and Susceptibility to Hepatocellular Carcinoma in a Chinese Population
Association Between Single Nucleotide Polymorphisms in miRNA196a-2 and miRNA146a and Susceptibility to Hepatocellular Carcinoma in a Chinese Population
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DOI：
10.7314/apjcp.2013.14.11.6427
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发表时间：
2013-01-01
影响因子：
--
通讯作者：
Liu, Jie
中科院分区：
文献类型：
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作者：
Zhang, Jun;Wang, Rui;Liu, Jie
肝细胞癌（HCC）是世界上最常见的癌症之一，严重威胁着人们的健康，尤其是在中国。早期诊断、预防和预测的技术仍在不断探索中，其中基于microRNA基因单核苷酸多态性（miRNA SNP）的方法是新提出的，并显示出潜在的潜力。特别是，已经研究了 miRNA196a-2 (rs11614913) 和 miRNA146a (rs2910164) 中的 SNP 与 HCC 之间的关联。然而，所得出的结论是相互矛盾的，可能是由于样本量不足或人口分层所致。仍需要在精心设计的大样本中进行进一步确认。在本研究中，我们通过 MassARRAY 检测在 2,000 个中国大型病例对照样本中验证了这两个 SNP 与 HCC 易感性之间的关联。 rs11614913 与 HCC 之间的显着关联已得到证实。 rs11614913中携带CT+TT或T等位基因基因型的受试者对HCC（CT+TT：OR（95% CI）=0.73（0.57-0.92），P=0.01；T等位基因：OR（95% CI）=0.85（0.75-0.97），P=0.02）和HBV相关HCC（CT+TT：OR (95% CI)=0.69 (0.53-0.90)，P=0.01；T 等位基因：OR (95% CI)=0.82 (0.71-0.95)，P=0.01)。 CT或TT受影响的携带者血清AFP水平也往往较低（P=0.01）。这项研究证明了 rs11614913 在 HCC 病因学中的作用。进一步的研究应集中于该miRNA SNP的临床应用，以助力攻克HCC。
Hepatocellular carcinoma (HCC) is one of the most prevalent cancers in the world and deeply threatens people's health, especially in China. Techniques of early diagnosis, prevention and prediction are still being discovered, among which the approaches based on single nucleotide polymorphisms in microRNA genes (miRNA SNPs) are newly proposed and show prospective potential. In particular, the association between SNPs in miRNA196a-2 (rs11614913) and miRNA146a (rs2910164) and HCC has been investigated. However, the conclusions made were conflicting, possibly due to insufficient sample size or population stratification. Further confirmations in well-designed large samples are still required. In this study, we verified the association between these two SNPs and the susceptibility to HCC by MassARRAY assay in a 2,000 large Chinese case-control sample. Significant association between rs11614913 and HCC was confirmed. Subjects with the genotype of CT+TT or T allele in rs11614913 were more resistant to HCC (CT+TT: OR (95% CI)=0.73 (0.57-0.92), P=0.01; T allele: OR (95% CI)=0.85 (0.75-0.97), P=0.02) and HBV-related HCC (CT+TT: OR (95% CI)=0.69 (0.53-0.90), P=0.01; T allele: OR (95% CI)=0.82 (0.71-0.95), P=0.01). The affected carriers of CT or TT also tended to have lower levels of serum AFP (P=0.01). This study demonstrated a role of rs11614913 in the etiology of HCC. Further research should focus on the clinical use of this miRNA SNP, so as to facilitate conquering HCC.