Changes of Western honey beeApis mellifera ligustica(Spinola, 1806) ventriculus microbial profile related to their in-hive tasks
Changes of Western honey beeApis mellifera ligustica(Spinola, 1806) ventriculus microbial profile related to their in-hive tasks
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DOI：
10.1080/00218839.2020.1830259
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发表时间：
2020-10-16
影响因子：
1.9
通讯作者：
Nanetti, Antonio
中科院分区：
文献类型：
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作者：
Cilia, Giovanni;Fratini, Filippo;Nanetti, Antonio
通过分析属于六种蜂巢内任务（新出现的蜜蜂、护士、造蜡工、守护者、年轻觅食者、年老觅食者）的蜜蜂，研究了蜂巢内任务对蜜蜂（Apis melliferaL.）心室微生物谱的影响。需氧菌总数、肠杆菌、葡萄球菌、大肠杆菌、乳酸菌、假单胞菌、需氧细菌内生孢子和肠球菌。使用基于培养的方法确定。所有培养的细菌均检测到显着的蜂巢内任务依赖性微生物特征 (p < 0.001)，其中新出现的细菌和造蜡剂的值最低，老觅食者的值最高。和葡萄球菌属。年轻觅食者的数量低于年老觅食者，而在其他调查的蜂巢内任务中则不存在。大肠杆菌和肠球菌属仅在年老的觅食者中检测到。 1 与其他蜂巢内任务相比，护士和老觅食者的乳酸菌含量较高，而新出现的乳酸菌含量最低。这项调查的结果表明，意大利蜜蜂的胃室微生物特征会根据蜂巢内的任务以及它们的年龄（对于觅食者而言）而发生变化。
The effects of in-hive tasks on the honey bee (Apis melliferaL.) ventriculus microbial profile were investigated by analysing honey bees belonging to six in-hive tasks (newly-emerged, nurses, wax builder, guardians, young foragers, old foragers). Total viable aerobic bacterial count, Enterobacteriaceae, staphylococci,Escherichia coli, lactic acid bacteria,Pseudomonasspp., aerobic bacterial endospores, andEnterococcusspp. were determined using a culture-based method. Significant in-hive tasks-dependent microbial profiles were detected for all cultured bacteria (p < 0.001), with the lowest values for newly-emerged and wax builder and the highest for old foragers.Pseudomonasspp. andStaphylococcusspp. were quantitatively lower in young foragers than in old foragers, while they were absent in the other investigated in-hive tasks.E. coliandEnterococcusspp. were detected only in old foragers. 1Lactic acid bacteria were higher in nurses and old foragers compared to the other in-hive tasks, while the newly-emerged showed the lowest amount of lactic acid bacteria. The results from this investigation showed that ventriculus microbiological profile ofApis mellifera ligusticaundergoes changes based on the in-hive task, and, as concerns foragers, on their age.