Cell-type-specific localization of transforming growth factor-beta 2 and transforming growth factor-beta 1 in the hamster ovary: differential regulation by follicle-stimulating hormone and luteinizing hormone.
Cell-type-specific localization of transforming growth factor-beta 2 and transforming growth factor-beta 1 in the hamster ovary: differential regulation by follicle-stimulating hormone and luteinizing hormone.
复制标题
仓鼠卵巢中转化生长因子-β 2 和转化生长因子-β 1 的细胞类型特异性定位：卵泡刺激素和黄体生成素的差异调节。
DOI：
10.1095/biolreprod46.4.595
复制
发表时间：
1992
影响因子：
3.6
通讯作者：
Beth Lu
中科院分区：
文献类型：
--
作者：
S. Roy;Christine Ogren;Chitrita Roy;Beth Lu
用免疫组织化学方法研究了3种条件下转化生长因子-β1(TGF-β1)和转化生长因子-β2(TGF-β2)在金黄地鼠卵巢中的细胞类型特异性定位和促性腺激素调节作用：(1)动情周期(第1天=发情期；第4天=发情前期)；(2)苯巴比妥阻断排卵期促性腺激素激增；卵巢转化生长因子-β1活性主要定位于膜细胞和间质细胞。促黄体生成素活性在排卵前促黄体生成素高峰期后略有升高，并在0900h、2h达到高峰，卵母细胞表现出相当的活性。术后4d，卵泡膜间质细胞中的转化生长因子-β1免疫反应降至较低水平，高峰后出现明显的转化生长因子-β2免疫反应，主要分布在腔前卵泡和腔前卵泡的颗粒细胞中，腔前卵泡周围有少量间质细胞呈散在染色。在腔前卵泡周围的颗粒细胞和间质细胞中，转化生长因子-β2免疫反应在第1天的0900小时达到高峰，并持续到第2天的09小时，卵母细胞未见染色。苯巴比妥治疗在1600h，即第4天，阻断了转化生长因子-β1和转化生长因子-β2免疫反应的出现；然而，在延迟1天后，随着高峰的出现，免疫反应开始反弹。长期应用促黄体生成素替代黄体生成素可显著增加间质细胞的转化生长因子-β1免疫反应活性，而促卵泡生成素虽能诱导卵泡发育，但不影响卵巢转化生长因子-β1的活性。用FSH处理后，新生腔和腔前卵泡颗粒细胞中的转化生长因子-β2免疫反应活性显著增加，而间质细胞中的转化生长因子-β2免疫反应不明显，而促黄体生成素对转化生长因子-β2的活性无明显影响。卵泡刺激素和促黄体生成素联合治疗后，颗粒细胞和间质细胞中的转化生长因子-β2免疫反应活性显著增加，膜细胞和间质细胞中的转化生长因子-β1的表达与完整动物的峰值活性相当。Western分析证实了转化生长因子-β1和转化生长因子-β2在金黄地鼠卵巢中的存在和免疫定位的特异性。因此，这些研究提供了重要的证据，证明转化生长因子-β1和转化生长因子-β2在特定的细胞类型中表达，并且它们的表达分别受黄体生成素和卵泡刺激素的不同调控。
Cell-type-specific localization and gonadotropin regulation of transforming growth factor-beta 1 (TGF-beta 1) and transforming growth factor-beta 2 (TGF-beta 2) in the hamster ovary were evaluated immunohistochemically under three conditions: (1) during the estrous cycle (Day 1 = estrus; Day 4 = proestrus); (2) after the blockade of periovulatory gonadotropin surges by phenobarbital, and (3) after FSH and/or LH treatment of long-term hypophysectomized hamsters. Ovarian TGF-beta 1 activity was primarily localized in theca and interstitial cells. The activity increased moderately but significantly after the preovulatory LH surge and reached a peak at 0900 h, Day 2 h; oocytes showed considerable activity. TGF-beta 1 immunoreactivity subsequently fell to low levels in theca-interstitial cells through 0900 h, Day 4. Significant TGF-beta 2 immunoreactivity appeared after the surge, mainly in the granulosa cells of both preantral and antral follicles; a few interstitial cells surrounding preantral follicles showed discrete staining. TGF-beta 2 immunoreactivity in granulosa cells and in interstitial cells next to preantral follicles reached a peak at 0900 h, Day 1, and persisted up to 0900 h, Day 2; oocytes showed no staining. Phenobarbital treatment blocked the appearance of TGF-beta 1 and TGF-beta 2 immunoreactivities at 1600 h, Day 4; however, a rebound in immunoreactivities was observed with the onset of the surge after a 1-day delay. Replacement of LH to long-term hypophysectomized hamsters resulted in a marked increase in TGF-beta 1 immunoreactivity in the interstitial cells, but FSH, although it induced follicular development, did not influence ovarian TGF-beta 1 activity. Treatment with FSH, however, induced a massive increase in TGF-beta 2 immunoreactivity in the granulosa cells of newly developed antral and preantral follicles but not in the interstitial cells; LH, on the other hand, had no significant effect on TGF-beta 2 activity. Treatment with FSH and LH combined resulted in a dramatic increase in TGF-beta 2 immunoreactivity in granulosa and interstitial cells and in TGF-beta 1 in theca and interstitial cells comparable to their peak activity in intact animals. Western analyses substantiated the presence of TGF-beta 1 and TGF-beta 2 in the hamster ovary and the specificity of immunolocalization. These studies, therefore, provide critical evidence that TGF-beta 1 and TGF-beta 2 in the hamster ovary are expressed in specific cell types and that their expression is differentially regulated by LH and FSH, respectively.
登录
查看更多内容
影响因子：
4.8
作者：
DODSON, WC;SCHOMBERG, DW
通讯作者：
SCHOMBERG, DW
卵巢膜细胞产生转化生长因子-β，可以调节颗粒细胞的生长。
影响因子：
4.8
作者：
Skinner,MK;Keski-Oja,J;Osteen,KG;Moses,HL
通讯作者：
Moses,HL
转化生长因子-β 1 抑制卵巢雄激素的产生：基因表达、细胞定位、机制和作用位点。
影响因子：
4.8
作者：
Hernandez,ER;Hurwitz,A;Payne,DW;Dharmarajan,AM;Purchio,AF;Adashi,EY
通讯作者：
Adashi,EY
大鼠颗粒细胞表达转化生长因子-β 2 型信使核糖核酸，该核糖核酸可在体外受卵泡刺激素调节。
影响因子：
4.8
作者：
Mulheron,GW;Schomberg,DW
通讯作者：
Schomberg,DW
影响因子：
4.8
作者：
E. Adashi;C. Resnick
通讯作者：
E. Adashi;C. Resnick