Exposure to a MRI-Type High-Strength Static Magnetic Field Stimulates Megakaryocytic/Erythroid Hematopoiesis in CD34+ Cells From Human Placental and Umbilical Cord Blood
Exposure to a MRI-Type High-Strength Static Magnetic Field Stimulates Megakaryocytic/Erythroid Hematopoiesis in CD34+ Cells From Human Placental and Umbilical Cord Blood
复制标题
DOI：
10.1002/bem.20480
复制
发表时间：
2009-05-01
影响因子：
1.9
通讯作者：
Kashiwakura, Ikuo
中科院分区：
文献类型：
--
作者：
Monzen, Satoru;Takahashi, Kenji;Kashiwakura, Ikuo
暴露于高强度静磁场（SMF）后的生物反应最近从可能的健康益处和潜在的不利影响的角度被广泛讨论。为了澄清这一问题，我们将来自人胎盘和脐带血的CD34细胞暴露在体外高强度SMF条件下。高强度SMF暴露系统由磁场发生器、无氦超导磁体和内置CO培养箱组成。将新制备的CD34细胞分别暴露于具有最强磁场梯度（41.7 T/m）的5特斯拉(T) SMF或无磁场梯度的10 T SMF中4小时或16小时。在暴露于10 T SMF 16小时后收获的细胞中，红细胞和巨核细胞祖细胞衍生的集落形成的总爆发单位中造血祖细胞的数量显著增加，分别比对照高1.72倍和1.77倍。此外，发现早期造血相关基因和细胞周期相关基因因暴露于SMF而显著上调。这些结果表明，10 T SMF暴露可能改变基因表达，导致巨核细胞/红细胞祖细胞（MEP）从多能造血干细胞分化和/或双能MEP增殖的特异性增强。(C) 2009 Wiley-Liss。公司。
The biological response after exposure to a high-strength static magnetic field (SMF) has recently been widely discussed from the perspective of possible health benefits as well as potential adverse effects. To clarify this issue, CD34 cells from human placental and Umbilical cord blood were exposed under conditions of high-strength SMF in vitro. The high-strength SMF exposure system was comprised of a magnetic field generator with a helium-free superconducting magnet with built-in CO, incubator. Freshly prepared CD34 cells were exposed to a 5 tesla (T) SMF with the strongest magnetic field gradient (41.7 T/m) or a 10 T SMF without magnetic field gradient for 4 or 16 h. In the harvested cells after exposure to 10 T SMF for 16 h, a significant increase of hematopoietic progenitors in the total burst-forming unit erythroid- and megakaryocytic progenitor cells-derived colony formation was observed, thus producing 1.72- and 1.77-fold higher than the control, respectively. Furthermore, early hematopoiesis-related and cell cycle-related genes were found to be significantly up-regulated by exposure to SMF. These results suggest that the 10 T SMF exposure may change gene expressions and result in the specific enhancement of megakaryocytic/erythroid progenitor (MEP) differentiation from pluripotent hematopoietic stem cells and/or the proliferation of bipotent MER Bioelectromagnetics 30:280-285, 2009. (C) 2009 Wiley-Liss. Inc.