Effect of cationic charge localization on DNA structure
Effect of cationic charge localization on DNA structure
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DOI：
10.1002/bip.10215
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发表时间：
2002-11-05
期刊：
影响因子：
2.9
通讯作者：
Gold, B
中科院分区：
文献类型：
--
作者：
Gold, B
DNA 通常被认为是刚性的棒状分子，但与 DNA 平衡结合或共价结合的蛋白质和小分子可以通过改变特定 DNA 序列处的局部疏水和静电环境来克服非线性障碍。蛋白质对 DNA 的变形已被证明是转录调控中的关键事件。在这篇综述中，我们重点关注阳离子电荷的引入及其位置如何影响 DNA 结构。为了研究电荷的影响，我们使用了在脱氧尿嘧啶的 5 位上经过 3-氨丙基取代修饰的 DNA，模拟碱性赖氨酸样侧链，并将阳离子电荷置于主沟中。先前对这些侧链的凝胶迁移率研究表明它们会使 DNA 弯曲。讨论了阳离子侧链的位置以及它们如何弯曲 DNA。 (C) 2002 年 Wiley 期刊公司
DNA is normally considered to be a stiff rod-like molecule, but proteins and small molecules, which either equilibrium bind to, or covalent bond with, DNA, can overcome the barrier(s) to non-linearity by changing the local hydrophobic and electrostatic environment at specific DNA sequences. The deformation of DNA by proteins has been shown to be a critical event in transcriptional regulation. In this review, we have focused on how the introduction of cationic charge, and its location, can affect DNA structure. To study the effect of charge, we have used DNA modified with 3-aminopropyl substitutions at the 5-position of deoxyuracil that mimic basic lysine-like sidechains, and which place the cationic charge in the major groove. Previous gel mobility studies with these sidechains have shown that they bend DNA. The location of the cationic sidechains and how they bend DNA is discussed. (C) 2002 Wiley Periodicals, Inc.