A second capsidless hadakavirus strain with 10 positive-sense single-stranded RNA genomic segments from Fusarium nygamai
A second capsidless hadakavirus strain with 10 positive-sense single-stranded RNA genomic segments from Fusarium nygamai
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DOI：
10.1007/s00705-021-05176-x
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发表时间：
2021-07
影响因子：
2.7
通讯作者：
H. Khan;Yukiyo Sato;H. Kondō;A. Jamal;M. F. Bhatti;N. Suzuki
中科院分区：
文献类型：
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作者：
H. Khan;Yukiyo Sato;H. Kondō;A. Jamal;M. F. Bhatti;N. Suzuki
一种独特的无衣壳病毒，具有正义、单链 RNA 基因组（哈达卡病毒 1，HadV1），是扩展的小核糖核酸样超群的成员，之前从植物病原真菌尖孢镰刀菌中分离出来。在这里，我们描述了来自镰刀菌的第二种哈达卡病毒株的分子和生物学特征，该病毒株之前尚未作为病毒宿主进行过详细研究。这种病毒，哈达卡病毒 1 株 1NL (HadV1-1NL)，与第一种哈达卡病毒 HadV1-7n 具有相似的特征，尽管其片段数量不同（HadV1-1NL 为 10 个，HadV1-7n 为 11 个）。 HadV1-1NL 的 10 个基因组 RNA 片段大小范围为 0.9 kb 至 2.5 kb。所有 HadV1-1NL 片段与其对应的 HadV1-7n 片段显示出 67% 至 86% 的局部核苷酸序列同一性，而 HadV1-1NL 没有编码锌指基序的 HadV1-7n RNA8 的同源物。另一个有趣的特征是 HadV1-1NL RNA10 可能无法编码。根据其复制形式 dsRNA 的 RNase A 敏感性，预计 HadV1-1NL 是无衣壳的。多种病毒感染和无病毒单孢子分离株的表型比较表明 HadV1-1NL 无症状感染。正如在 HadV1-7n 中观察到的那样，随着孢子所源自的受感染真菌菌落变老，通过孢子的垂直传播效率较低。这项研究展示了哈达卡病毒的第二个例子，它似乎具有不寻常的特征。
A unique capsidless virus with a positive-sense, single-stranded RNA genome (hadakavirus 1, HadV1), a member of the extended picorna-like supergroup, was isolated previously from the phytopathogenic fungusFusarium oxysporum. Here, we describe the molecular and biological characterisation of a second hadakavirus strain fromFusarium nygamai, which has not been investigated in detail previously as a virus host. This virus, hadakavirus 1 strain 1NL (HadV1-1NL), has features similar to the first hadakavirus, HadV1-7n, despite having a different number of segments (10 for HadV1-1NL vs. 11 for HadV1-7n). The 10 genomic RNA segments of HadV1-1NL range in size from 0.9 kb to 2.5 kb. All HadV1-1NL segments show 67% to 86% local nucleotide sequence identity to their HadV1-7n counterparts, whereas HadV1-1NL has no homolog of HadV1-7n RNA8, which encodes a zinc-finger motif. Another interesting feature is the possible coding incapability of HadV1-1NL RNA10. HadV1-1NL was predicted to be capsidless based on the RNase A susceptibility of its replicative form dsRNA. Phenotypic comparison of multiple virus-infected and virus-free single-spore isolates indicated asymptomatic infection by HadV1-1NL. Less-efficient vertical transmission via spores was observed as the infected fungal colonies from which the spores were derived became older, as was observed for HadV1-7n. This study shows a second example of a hadakavirus that appears to have unusual features.