Effect of vitamin C, deferoxamine, quercetin and rutin against tert-butyl hydroperoxide oxidative damage in human erythrocytes
Effect of vitamin C, deferoxamine, quercetin and rutin against tert-butyl hydroperoxide oxidative damage in human erythrocytes
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DOI：
10.1179/102453309x402296
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发表时间：
2009-06-01
期刊：
影响因子：
1.9
通讯作者：
do Nascimento, Aguinaldo Jose
中科院分区：
文献类型：
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作者：
Krukoski, Daniel Witchmichen;Comar, Samuel Ricardo;do Nascimento, Aguinaldo Jose
成熟的人红细胞，当受到氧化应激时，可以表现出还原性谷胱甘肽的消耗，血红蛋白分子的氧化和靠近膜的铁复合物的聚集。这些可导致红细胞膜异常和溶血。本研究的目的是研究维生素C的抗氧化作用。C)，脱铁胺（DFO）和黄酮类槲皮素和芦丁在正常的人红细胞，提交了体外氧化应激的叔丁基过氧化氢（(BHP)-B-t）。经知情同意后，健康成人静脉血以柠檬酸盐-磷酸盐-葡萄糖（CPD）溶液作为抗凝剂采集。将红细胞在PBS中重悬以获得35%的球形体积，然后将其置于(BHP)-B-t的氧化作用下长达30分钟，之前用vit孵育或不孵育60分钟。C， DFO，槲皮素和芦丁。随着(BHP)-B-t浓度的增加，GSH浓度、G6-PD和GR活性降低，高铁血红蛋白和亨氏小体（HB）形成增加。（BHP）-B-t对SDS-PAGE检测的膜蛋白无影响。槲皮素部分阻止GSH的降低和HB的形成，但对(BHP)-B-t氧化损伤MetHb的形成没有作用。芦丁在(BHP)-B-t诱导后，抑制了GSH的降低和HB的形成。维特。（BHP）-B-t对GSH的消耗无影响，部分抑制metHb的形成，对GR有保护作用，但对G6-PD无保护作用。DFO部分抑制metHb的形成和GSH的降低，但对(BHP)-B-t对GR和G6-PD的氧化损伤无保护作用。所得结果表明，该方法是有效的。C、DFO和黄酮类化合物槲皮素和芦丁均有助于减轻(BHP)-B-t引起的氧化应激。
The mature human erythrocyte, when submitted to oxidative stress, can demonstrate depletion of reduced glutathione, oxidation of the hemoglobin molecule and aggregation of complexes of iron close to the membrane. These can produce abnormalities in the erythrocyte membrane and hemolysis. The aim of this work was to study the antioxidative action of vitamin C ( vit. C), deferroxamine ( DFO) and the flavonoids quercetin and rutin in normal human erythrocytes, submitted to in vitro oxidative stress induced by tert-butylhydroperoxide ( (BHP)-B-t). Venous blood was colleted in citrate-phosphate-dextrose ( CPD) solution, as anticoagulant, from healthy adult individuals after informed consent. The erythrocytes were resuspended in PBS to obtain 35% globular volume, and then submitted to the oxidative action of (BHP)-B-t for up to 30 min, with or without previous incubation for 60 min with vit. C, DFO, quercetin and rutin. Decrease in the GSH concentration, G6-PD and GR activities, and increase in the methemoglobin and Heinz bodies ( HB) formation, occurred with the increase in (BHP)-B-t concentration. (BHP)-B-t did not effect on the membrane proteins detected by SDS-PAGE. Quercetin, partially prevented the GSH decrease and the formation of HB, but did not prevent MetHb formation from oxidative damage by (BHP)-B-t. Rutin, after (BHP)-B-t induction, prevented the GSH decrease and the formation of HB. Vit. C, had no influence on the depletion of GSH, inhibited partially the metHb formation, and it protected GR, but not G6-PD from oxidative damage by (BHP)-B-t. DFO partially inhibited the metHb formation and GSH decrease, but it did not protect GR and G6-PD from oxidative damage by (BHP)-B-t. The results obtained suggest that vit. C, DFO and the flavonoids quercetin and rutin contribute to the decrease in the oxidative stress caused by (BHP)-B-t.