A low molecular weight isoform of hyaluronidase:: Purification from Indian cobra (Naja naja) venom and partial characterization
A low molecular weight isoform of hyaluronidase:: Purification from Indian cobra (Naja naja) venom and partial characterization
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DOI：
10.1007/s10541-005-0172-6
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发表时间：
2005-06-01
影响因子：
2.8
通讯作者：
Kemparaju, K
中科院分区：
文献类型：
--
作者：
Girish, KS;Kemparaju, K
通过在 Sephadex G-75 和 CM-Sephadex C-25 柱上连续层析，从印度眼镜蛇 (Naja naja) 毒液中分离出低分子量透明质酸酶 (NNH2) 亚型。 SDS-PAGE测定表观分子量为52 kD，pI值为9.7。 NNH2 是一种糖苷内切酶，在体外对透明质酸表现出绝对特异性；它还可以水解人体皮肤切片中的透明质酸。 NNH2 无毒，但它间接增强出血复合物-I 的出血活性。姜黄素、吲哚美辛和单宁酸可剂量依赖性地抑制 NNH2 对乙酰透明质酸的降解。
A low molecular weight isoform of hyaluronidase (NNH2) has been isolated from Indian cobra (Naja naja) venom by successive chromatography on Sephadex G-75 and CM-Sephadex C-25 columns. The apparent molecular weight determined by SDS-PAGE is 52 kD, and the pI value is 9.7. NNH2 is an endoglycosidase and exhibits in vitro absolute specificity for hyaluronan; it also hydrolyzed hyaluronan in human skin sections. NNH2 is nontoxic, but it indirectly potentiates the hemorrhagic activity of hemorrhagic complex-I. Curcumin, indomethacin, and tannic acid inhibited dose dependently the degradation of hyaluronan by NNH2.