Specific enrichment of the suppressor T cell bearing I-J determinants: parallel functional and serological characterizations.
Specific enrichment of the suppressor T cell bearing I-J determinants: parallel functional and serological characterizations.
复制标题
具有 I-J 决定簇的抑制性 T 细胞的特异性富集：平行功能和血清学特征。
DOI：
10.1084/jem.146.5.1234
复制
发表时间：
1977-11-01
影响因子：
15.3
通讯作者：
Tada, T
中科院分区：
文献类型：
--
作者：
Okumura, K;Takemori, T;Tokuhisa, T;Tada, T
A simple procedure to enrich the antigen (keyhole limpet hemocyanin, KLH)-specific suppressor T cell was described. The suppressor T cell from KLH)-immunized mice specifically bound to the KLH-coated Sephadex G-200 column at 37 degrees C, and was eluted from the column by cold (0- 4 degrees C) medium. The helper T cell did not bind to the column under the identical condition. The suppressor T cell thus obtained had 100 times as potent suppressor activity as the original spleen cells in in vivo and in vitro secondary antibody responses against a hapten coupled to KLH. This procedure also enriched the cells bearing I-J determinants and Lyt-2,3 alloantigens, allowing us to study the phenotypic expressions on the suppressor T cell by direct serological procedures as well as by the use of the fluorescence activated cell sorter. Parallel functional and serological analyses indicated that the antigen- specific suppressor T cell belongs to a population of I-J+, Lyt-2+,3+ and Fc R- T cells.
