Phenobarbital induction of cytochrome p‐450 b,e genes is dependent on protein synthesis
Phenobarbital induction of cytochrome p‐450 b,e genes is dependent on protein synthesis
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苯巴比妥对细胞色素 p-450 b,e 基因的诱导依赖于蛋白质合成
DOI：
10.1002/hep.1840080223
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发表时间：
1988
期刊：
影响因子：
13.5
通讯作者：
J. Gumucio
中科院分区：
文献类型：
--
作者：
J. Chianale;L. Mulholland;P. Traber;J. Gumucio
Phenobarbital induces liver cytochrome P‐450 b,e proteins mainly by increasing the rate of transcription of these genes. The mechanism responsible for the phenobarbital increment in the rate of transcription of cytochrome P‐450 b,e genes is unknown. The objective of this study was to assess whether active protein synthesis was needed for phenobarbital to induce the liver cytochrome P‐450 b,e genes. Cycloheximide (2 mg per kg, i.p.) was administered 90 min prior to a single inductive dose of phenobarbital (80 mg per kg, i.p.) and mRNAS measured at 3, 6 and 12 hr by dot‐blot hybridization. While phenobarbital increased cytochrome P‐450 b,e mRNAs about 12‐fold at 3 hr, this induction was abolished by cycloheximide. To define whether the absence of protein synthesis in hepatocytes inhibited the phenobarbital induction of cytochrome P‐450 at the transcriptional level, in vitro transcription rates using isolated nuclei were measured. After phenobarbital administration, there was about a 20‐fold increment in transcriptional rate of cytochrome P‐450 b,e genes. This increment was abolished by prior injection of cycloheximide. It is proposed that either preexisting regulatory proteins or transacting factors dependent on active protein synthesis participate in the regulation of liver cytochrome P‐450 b,e gene transcription after phenobarbital.
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影响因子：
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DOI：
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发表时间：
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期刊：
The Journal of biological chemistry
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