Identification of urinary metabolites of the red clover isoflavones formononetin and biochanin A in human subjects
Identification of urinary metabolites of the red clover isoflavones formononetin and biochanin A in human subjects
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DOI：
10.1021/jf0492767
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发表时间：
2004-11-03
影响因子：
6.1
通讯作者：
Adlercreutz, H
中科院分区：
文献类型：
--
作者：
Heinonen, SM;Wähälä, K;Adlercreutz, H
由红三叶草制成的膳食补充剂被广泛销售，以提供有益的健康效果，而不改变原来的饮食。在这项研究中，芒柄花素和鹰嘴豆芽素A，红三叶草的主要生物碱，在人体中的代谢进行了研究。七名妇女摄入了四种红三叶草膳食补充剂，并在他们的尿液样本中鉴定出了这些化合物的代谢产物。通过GC-MS确定了代谢物的三甲基硅烷基衍生物的结构。使用真实的参比化合物在尿液样本中鉴定了新的还原代谢物芒柄花素（二氢芒柄花素和安哥拉辛）和鹰嘴豆芽素A（二氢鹰嘴豆芽素A和6 '-羟基安哥拉辛）。提出了红三叶草黄酮芒柄花素和鹰嘴豆芽素A的可能代谢途径。
Dietary supplements manufactured from red clover are widely marketed to provide the beneficial health effects of isoflavones without changing the original diet. In this study the metabolism of formononetin and biochanin A, the principal isoflavones of red clover, was studied in human subjects. Seven women ingested four red clover dietary supplements, and the metabolites of the isoflavones were identified in their urine samples. The structures of trimethylsilyl derivatives of the metabolites were established by GC-MS. New reduced metabolites of formononetin (dihydroformononetin and angolensin) and biochanin A (dihydrobiochanin A and 6'-hydroxyangolensin) were identified in urine samples using authentic reference compounds. Possible metabolic pathways are presented for the red clover isoflavones formononetin and biochanin A.