Long-term single-cell imaging of mammalian stem cells
Long-term single-cell imaging of mammalian stem cells
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DOI：
10.1038/nmeth.1577
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发表时间：
2011-04-01
期刊：
影响因子：
48
通讯作者：
Schroeder, Timm
中科院分区：
文献类型：
--
作者：
Schroeder, Timm
连续长期的单细胞观察提供了对细胞命运的分子控制的见解。这对于罕见的异质细胞群，如哺乳动物干细胞，尤其重要。目前缺乏可用于此类实验的现成硬件和软件，这使得它们的实现在技术上具有挑战性。在这里，我讨论了对连续单细胞定量的需求，以了解分子细胞命运控制，以及长期成像和跟踪干细胞的组织和技术解决方案。
Continuous long-term single-cell observation provides insight into the molecular control of cell fate. This is particularly important for rare and heterogeneous populations of cells, such as mammalian stem cells. The current lack of usable off-the-shelf hardware and software for such experiments makes their implementation technically challenging. Here I discuss the need for continuous single-cell quantification to understand molecular cell fate control as well as organizational and technical solutions for long-term imaging and tracking of stem cells.