In vivo imaging with antibodies and engineered fragments.
In vivo imaging with antibodies and engineered fragments.
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DOI：
10.1016/j.molimm.2015.04.001
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发表时间：
2015-10
影响因子：
3.6
通讯作者：
Wu AM
中科院分区：
文献类型：
--
作者：
Freise AC;Wu AM
抗体已经清楚地证明了它们作为治疗手段的效用，为治疗肿瘤学、血液学、心脏病学、免疫学和自身免疫以及感染性疾病提供了高度选择性和有效的药物。最近，迫切需要具有同等特异性和针对性的显像剂来评估临床前模型和患者体内的疾病。本文综述了开发和优化抗体作为靶向探针用于放射性、光学、磁共振和超声等非侵入性成像的策略。重点介绍了工程抗体片段和支架、偶联和标记方法以及多模态探针的最新进展。重要的是，基于抗体的成像探针在细胞表面生物标记物的检测和定量、对靶向治疗的特异性反应成像以及肿瘤和其他疾病的免疫反应监测方面有了新的应用。基于抗体的成像将为促进向真正精准医疗的过渡提供必要的工具。
Antibodies have clearly demonstrated their utility as therapeutics, providing highly selective and effective drugs to treat diseases in oncology, hematology, cardiology, immunology and autoimmunity, and infectious diseases. More recently, a pressing need for equally specific and targeted imaging agents for assessing disease in vivo, in preclinical models and patients, has emerged. This review summarizes strategies for developing and optimizing antibodies as targeted probes for use in non-invasive imaging using radioactive, optical, magnetic resonance, and ultrasound approaches. Recent advances in engineered antibody fragments and scaffolds, conjugation and labeling methods, and multimodality probes are highlighted. Importantly, antibody-based imaging probes are seeing new applications in detection and quantitation of cell surface biomarkers, imaging specific responses to targeted therapies, and monitoring immune responses in oncology and other diseases. Antibody-based imaging will provide essential tools to facilitate the transition to truly precision medicine.