Use of lambda pMu bacteriophages to isolate lambda specialized transducing bacteriophages carrying genes for bacterial chemotaxis.
Use of lambda pMu bacteriophages to isolate lambda specialized transducing bacteriophages carrying genes for bacterial chemotaxis.
复制标题
使用 lambda pMu 噬菌体分离携带细菌趋化基因的 lambda 专门转导噬菌体。
DOI：
10.1128/jvi.35.3.619-628.1980
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发表时间：
1980
影响因子：
5.4
通讯作者：
Howe,MM
中科院分区：
文献类型：
--
作者：
Kondoh,H;Paul,BR;Howe,MM
描述了一种构造lambda专用转换器的一般方法。该方法，这是潜在的适用于任何基因的大肠杆菌，是基于使用Mu DNA同源性，以指导附近的基因，其转导是所需的λ pMu噬菌体的整合。用这种方法，我们分离了携带che基因簇中所有10个基因的λ转导噬菌体（图谱位置，41.5至42.5分钟）。cheA和tar基因的产物通过使用在这些基因中具有琥珀突变的转导引物来鉴定。已确定tar编码甲基接受趋化蛋白II（分子量62，000），cheA编码两种多肽（分子量76，000和66，000）。两个cheA多肽的可能起源进行了讨论。
A general method for constructing lambda specialized transducing phages is described. The method, which is potentially applicable to any gene of Escherichia coli, is based on using Mu DNA homology to direct the integration of a lambda pMu phage near the genes whose transduction is desired. With this method we isolated a lambda transducing phage carrying all 10 genes in the che gene cluster (map location, 41.5 to 42.5 min). The products of the cheA and tar genes were identified by using transducing phages with amber mutations in these genes. It was established that tar codes for methyl-accepting chemotaxis protein II (molecular weight, 62,000) and that cheA codes for two polypeptides (molecular weights, 76,000 and 66,000). Possible origins of the two cheA polypeptides are discussed.