Optimisation of the inactivated vaccine dose against heartwater and in vitro quantification of Ehrlichia ruminantium challenge material.
Optimisation of the inactivated vaccine dose against heartwater and in vitro quantification of Ehrlichia ruminantium challenge material.
复制标题
针对心水的灭活疫苗剂量的优化以及反刍埃里希体攻击材料的体外定量。
DOI：
10.1016/j.vaccine.2006.03.031
复制
发表时间：
2006
期刊：
影响因子：
5.5
通讯作者：
D. Martinez
中科院分区：
文献类型：
--
作者：
N. Vachiéry;T. Lefrançois;I. Esteves;S. Molia;C. Sheikboudou;Y. Kandassamy;D. Martinez
这项工作的目的是评估灭活疫苗对心水的最小保护剂量。为了进行可靠的疫苗接种试验，建立了反刍埃立克体（ER）攻毒剂量的体外定量方法。在实验条件下，用2.1× 104 ~ 9.4× 104个活ER原体进行的同源Gardel攻击是可重复的，并模仿了自然攻击。用5个不同ER株的3× 104个活原体攻击动物，也得到类似的结果。与常规剂量的灭活疫苗相比，疫苗剂量减少28倍不会降低保护作用。两次注射35μg的ER抗原诱导了针对心水的良好保护。此外，我们发现在体外用ER抗原刺激后，免疫动物血液中分泌的IFNγ的量不是存活的可靠预测因子，并且不能用于检测灭活疫苗的效力。
The aim of this work was to evaluate the minimal protective dose of the inactivated vaccine against heartwater. In order to conduct a reliable vaccination trial, an in vitro quantification method of Ehrlichia ruminantium (ER) challenge doses was developed. In experimental conditions, homologous Gardel challenges with 2.1×104to 9.4×104live ER elementary bodies were reproducible and mimicked a natural challenge. Similar results were obtained when animals were challenged with 3×104live elementary bodies from five different ER strains. A 28-fold reduction of the vaccine dose did not decrease protection when compared to the conventional dose of inactivated vaccine. Two injections of 35μg of ER antigen induced good protection against heartwater. Moreover, we found that the amount of IFNγ secreted in blood from vaccinated animals after in vitro stimulation with ER antigen was not a reliable predictor of survival and could not be used to test the potency of the inactivated vaccine.