Characterization of heat shock protein 70 gene from Haemonchus contortus and its expression and promoter analysis in Caenorhabditis elegans
Characterization of heat shock protein 70 gene from Haemonchus contortus and its expression and promoter analysis in Caenorhabditis elegans
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捻转血矛线虫热休克蛋白70基因的鉴定及其在秀丽隐杆线虫中的表达和启动子分析
DOI：
10.1017/s0031182012002168
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发表时间：
2013-01
期刊：
影响因子：
2.4
通讯作者：
AIFANG DU
中科院分区：
文献类型：
--
作者：
HONGLI ZHANG;QIANJIN ZHOU;YI YANG;XUEQIU CHEN;BAOLONG YAN;AIFANG DU
在世界范围内，小反刍动物中的弯曲血蜱感染具有重要的经济意义。热休克蛋白（HSPs）是一类分子伴侣蛋白，在线虫的入侵和生存过程中起重要作用。虽然HSP70已在几种寄生线虫中被发现，但对其在弯曲血蜱中的分布和功能知之甚少。本研究的目的是表征弯曲血蜱HSP70（设计为Hc-hsp70），表达Hc-hsp70并分析其在秀丽隐杆线虫中的启动子活性。生物信息学分析显示，Hc-hsp70 cDNA的开放阅读框编码一个646个氨基酸的肽，与其他线虫的HSP70相比具有高度保守性。系统发育分析表明，弓形虫与隐杆线虫有密切的亲缘关系。5 ' -侧翼区促进了线虫幼虫和成虫肠道中绿色荧光蛋白（GFP）的表达，在线虫中有两种表达模式。Hc-hsp70 mRNA转录本在秀丽隐杆线虫热休克2、4、6 h后表达增加，并在4 h达到峰值，但其表达可诱导秀丽隐杆线虫热休克1的下调。这些结果表明，弯螺旋体hsp70可能具有与秀丽隐杆线虫hsp-1相似的功能。
SUMMARY Haemonchus contortus infections in small ruminants are of major economic importance worldwide. Heat shock proteins (HSPs) are a family of molecular chaperones that play important roles in the process of invasion and survival of nematodes. Although HSP70 has been identified in several parasitic nematodes, little is known of its distribution and function in Haemonchus contortus. The aims of this study were to characterize HSP70 from Haemonchus contortus (designed as Hc-hsp70), express Hc-hsp70 and analyse the promoter activity in Caenorhabditis elegans. Bioinformatic analysis revealed that the open reading frame of the Hc-hsp70 cDNA encodes a 646-amino acid peptide, which is highly conserved in comparison to HSP70 in other nematodes. Phylogenetic analysis indicated that H. contortus is closely related to Caenorhabditis. The 5′-flanking region promoted green fluorescence protein (GFP) expression in the intestine in all larval stages and adult with 2 expression patterns in C. elegans. Expression of Hc-hsp70 mRNA transcripts in C. elegans increased following 2, 4, 6 h of heat shock and peaked at 4 h. However, its expression induced down-regulation of hsp-1 of C. elegans. These results suggest that the H. contortus hsp70 might have a similar function to that of C. elegans hsp-1.
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影响因子：
4.4
作者：
M. Selkirk;D. Denham;F. Partono;R. Maizels
通讯作者：
M. Selkirk;D. Denham;F. Partono;R. Maizels
DOI：
10.1002/9781119432401.ch9
发表时间：
2020-02
期刊：
Heat Stress Tolerance in Plants
影响因子：
--
作者：
Annu Yadav;Jitender Singh;K. Ranjan;Pankaj Kumar;Shivani Khanna;Madhuri Gupta;Vinay Kumar;S. H. Wani;Anil Sirohi
通讯作者：
Annu Yadav;Jitender Singh;K. Ranjan;Pankaj Kumar;Shivani Khanna;Madhuri Gupta;Vinay Kumar;S. H. Wani;Anil Sirohi
旋毛虫热休克蛋白 70 的分子克隆和表征。
影响因子：
2.7
作者：
Wang, Shaohua;Zhu, Xinping;Cui, Shijuan
通讯作者：
Cui, Shijuan
影响因子：
4
作者：
Hu M;Lok JB;Ranjit N;Massey HC Jr;Sternberg PW;Gasser RB
通讯作者：
Gasser RB
影响因子：
1.7
作者：
J. Dzik
通讯作者：
J. Dzik