Benzoyl-arginine naphthylamide (BANA) hydrolysis by Treponema denticola and/or Bacteroides gingivalis in periodontal plaques.
Benzoyl-arginine naphthylamide (BANA) hydrolysis by Treponema denticola and/or Bacteroides gingivalis in periodontal plaques.
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牙周菌斑中的齿垢密螺旋体和/或牙龈拟杆菌对苯甲酰精氨酸萘酰胺 (BANA) 进行水解。
DOI：
10.1111/j.1399-302x.1990.tb00425.x
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发表时间：
1990
影响因子：
--
通讯作者：
Loesche,WJ
中科院分区：
文献类型：
--
作者：
Bretz,WA;Lopatin,DE;Loesche,WJ
齿垢密螺旋体和牙龈拟杆菌是在牙周袋中发现的少数公认的可以水解合成肽N-苯甲酰基-DL-精氨酸-2-萘胺（BANA）的物种。我们通过使用间接免疫荧光抗体技术确定了BANA阳性和阴性菌斑样本中这些牙周病原体的存在。27个病变部位中有18个BANA阳性反应，9个BANA阴性反应。在18个BANA阳性反应中有16个存在牙垢，而在18个BANA阳性反应中有9个检测到牙龈炎。1例和B. 9例BANA阴性反应中有2例为牙龈菌素。在BANA阴性的19个健康部位中均未检出微生物。在相同个体中获得的BANA阳性和BANA阴性斑块之间的所有测量差异均具有统计学显著性。BANA测试的准确性与临床参数（如探查时出血和探查深度增加）相比约为80%。该试验检测T. B组为93%。牙龈炎76%; T. denticola和/或B.牙龈炎96%。这项研究表明，BANA阳性斑块与T的存在有关。denticola和/或B. gingivalis，即T.在BANA阳性斑块中，发现齿状回的频率和水平高于B。牙龈炎，并且这些生物体的存在与临床疾病相关。
Treponema denticola and Bacteroides gingivalisare among the few recognized species found in periodontal pockets that can hydrolyze the synthetic peptide N‐benzoyI‐DL‐arginine‐2‐naphthylamide (BANA). We determined the presence of these periodontal pathogens in BANA‐positive and ‐negative plaque samples through the use of indirect immunofluorescence antibody techniques. Eighteen of 27 diseased sites gave BANA‐positive reactions, and 9 gave BANA‐negative reactions.T. denticolawas present in 16 of 18 BANA‐positive reactions, whereas & gingivalis was detected in 9 of the 18 BANA‐positive reactions.T. denticolawas present in 1 andB. gingivalisin 2 of the 9 BANA‐negative reactions. Neither Organism was detected in the 19 healthy sites that were negative for BANA. All Measured differences between BANA‐positive and BANA‐negative plaques obtained in the same individuals were statistically significant. The accuracy of the BANA test, compared with clinical parameters such as bleeding upon probing and increased probing depth, was about 80%. The accuracy of the test in detecting the presence ofT. denticolawas 93%, for B. gingivalis. 76% and forT. denticolaand/orB. gingivalis, 96%. This study indicated that BANA‐positive plaques were associated with the presence ofT. denticolaand/orB. gingivalis, thatT. denticolaWas found at a greater frequency and levels in BANA‐positive plaques thanB. gingivalis, and that the presence of these organisms was associated with clinical disease.