A novel CCCH-type zinc finger protein SAW1 activates OsGA20ox3 to regulate gibberellin homeostasis and anther development in rice
A novel CCCH-type zinc finger protein SAW1 activates OsGA20ox3 to regulate gibberellin homeostasis and anther development in rice
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DOI：
10.1111/jipb.12924
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发表时间：
2020-04-10
影响因子：
11.4
通讯作者：
Zhu, Qinlong
中科院分区：
文献类型：
--
作者：
Wang, Bin;Fang, Ruiqiu;Zhu, Qinlong
雄性不育是杂交种子生产的先决条件。植物激素赤霉素 (GA) 参与调节雄性生殖发育，但花药发育中 GA 稳态的机制仍知之甚少。在这里，我们报告了一种新的 GA 稳态正调节因子——肿胀花药壁 1（SAW1）的分离和表征，该调节因子用于水稻（Oryza sativaL.）花药发育。携带隐性突变体等位基因锯1的水稻植株会产生异常花药，花药壁肿胀，花粉败育。水稻中成簇规则间隔短回文重复序列（CRISPR）/CRIPSR相关蛋白9介导的SAW1敲除产生了相似的雄性不育植株。SAW1编码一种新型的核定位CCCH串联锌指蛋白，该蛋白可以直接与GA合成基因OsGA20ox3的启动子区结合，诱导其花药特异性表达。在saw1花药中，OsGA20ox3表达显着降低，导致生物活性GA含量降低，进而导致GA诱导的花药调节基因OsGAMYB表达降低。因此，我们的研究结果揭示了SAW1-GA20ox3-GAMYB途径控制水稻花药发育的机制，为通过基因组编辑快速产生雄性不育系杂交育种提供了新的靶基因。
Male sterility is a prerequisite for hybrid seed production. The phytohormone gibberellin (GA) is involved in regulating male reproductive development, but the mechanism underlying GA homeostasis in anther development remains less understood. Here, we report the isolation and characterization of a new positive regulator of GA homeostasis,swollen anther wall 1(SAW1), for anther development in rice (Oryza sativaL.). Rice plants carrying the recessive mutant allelesaw1produces abnormal anthers with swollen anther wall and aborted pollen. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRIPSR-associated protein 9-mediated knockout ofSAW1in rice generated similar male sterile plants.SAW1encodes a novel nucleus-localizing CCCH-tandem zinc finger protein, and this protein could directly bind to the promoter region of the GA synthesis geneOsGA20ox3to induce its anther-specific expression. In thesaw1anther, the significantly decreasedOsGA20ox3expression resulted in lower bioactive GA content, which in turn caused the lower expression of the GA-inducible anther-regulator geneOsGAMYB. Thus, our results disclose the mechanism of theSAW1-GA20ox3-GAMYBpathway in controlling rice anther development, and provide a new target gene for the rapid generation of male sterile lines by genome editing for hybrid breeding.