Time-dependent effect of pneumonectomy on alveolar epithelial fluid clearance in rat lungs.
Time-dependent effect of pneumonectomy on alveolar epithelial fluid clearance in rat lungs.
复制标题
全肺切除术对大鼠肺肺泡上皮液清除率的时间依赖性影响。
DOI：
10.1067/mtc.2002.122549
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
Matthay,MichaelA
中科院分区：
文献类型：
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作者：
Sakuma,Tsutomu;Sagawa,Motoyasu;Hida,Mieko;Nambu,Yoshihiro;Osanai,Kazuhiro;Toga,Hirohisa;Takahashi,Keiji;Ohya,Nobuo;Matthay,MichaelA
由于肺切除术启动代偿性生长的剩余肺，我们确定了肺切除术对肺泡液体清除capacity.MethodsAlveolar液体清除能力与伊文思蓝标记的白蛋白浓度的时间依赖性影响，在大鼠左肺切除术后3小时，2天，7天，14天，28天。负责肺泡液体清除率增加的机制进行了explored.ResultsAlveolar液体清除率在剩余的肺是正常的，通过7天，然后增加14和28天后肺切除术。肺切除术后28天肺泡液体清除率的增加主要是由于阿米洛利敏感性转运的增加。上皮钠通道信使RNA的表达增加，其余的肺和II型肺泡上皮细胞分离大鼠肺切除术后28天。肺切除大鼠中分离的II型细胞的数量大于对照大鼠。此外，β-肾上腺素能受体激动剂治疗增加了肺泡液体清除率在3小时和28天的时间点。ConclusionsThe能力，以消除肺泡液体在剩余的肺是维持在一个正常的水平长达7天后，肺切除术在大鼠，然后有一个显着增加阿米洛利敏感的肺泡液体运输能力，这可能取决于，至少部分，在II型细胞中增加上皮钠通道的表达和部分地增加II型细胞的数量。
ObjectiveBecause pneumonectomy initiates compensatory growth of the remaining lung, we determined the time-dependent effects of pneumonectomy on alveolar fluid clearance capacity.MethodsAlveolar fluid clearance capacity with the Evans blue-labeled albumin concentration was measured in rats 3 hours, 2 days, 7 days, 14 days, and 28 days after left pneumonectomy. The mechanisms responsible for the increase in alveolar fluid clearance were explored.ResultsAlveolar fluid clearance in the remaining lung was normal through 7 days and then increased 14 and 28 days after pneumonectomy. The increase in alveolar fluid clearance at 28 days after pneumonectomy was accounted primarily by an increase in amiloride-sensitive transport. The expression of epithelial sodium channel messenger RNA was increased in the remaining lung and in type II alveolar epithelial cells isolated from rats 28 days after pneumonectomy. The number of isolated type II cells was larger in pneumonectomized rats than in control rats. Also, β-adrenergic agonist therapy increased the rate of alveolar fluid clearance at the 3-hour and 28-day time points.ConclusionsThe capacity to remove alveolar fluid in the remaining lung is maintained at a normal level for up to 7 days after pneumonectomy in a rat, and then there is a marked increase in amiloride-sensitive alveolar fluid transport capacity that might depend, at least in part, on increased expression of epithelial sodium channels in type II cells and in part on the increased number of type II cells.