Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models.
Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models.
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DOI：
10.1038/ncomms15028
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发表时间：
2017-04-11
影响因子：
16.6
通讯作者：
Chang MW
中科院分区：
文献类型：
--
作者：
Hwang IY;Koh E;Wong A;March JC;Bentley WE;Lee YS;Chang MW
细菌可以通过基因工程来杀死特定的病原体或抑制它们的毒性。我们之前开发了一种合成遗传系统，使大肠杆菌实验室菌株能够在体外感知并杀死铜绿假单胞菌。在这里，我们生成了一个修改版本的系统，包括一个编码抗生物膜酶的基因，并使用益生菌菌株大肠杆菌Nissle 1917作为宿主。在两种动物模型（秀丽隐杆线虫和小鼠）的肠道感染中，工程益生菌显示出体内对铜绿假单胞菌的预防和治疗活性。这些发现支持进一步开发具有潜在预防和治疗肠道感染活性的工程微生物。细菌可以被改造来杀死特定的病原体。在此，作者修改并优化了大肠杆菌益生菌菌株的合成遗传系统，并在两种肠道感染动物模型中证明了工程益生菌可以感知并杀死病原体铜绿假单胞菌。
Bacteria can be genetically engineered to kill specific pathogens or inhibit their virulence. We previously developed a synthetic genetic system that allows a laboratory strain of Escherichia coli to sense and kill Pseudomonas aeruginosa in vitro. Here, we generate a modified version of the system, including a gene encoding an anti-biofilm enzyme, and use the probiotic strain Escherichia coli Nissle 1917 as host. The engineered probiotic shows in vivo prophylactic and therapeutic activity against P. aeruginosa during gut infection in two animal models (Caenorhabditis elegans and mice). These findings support the further development of engineered microorganisms with potential prophylactic and therapeutic activities against gut infections. Bacteria can be engineered to kill specific pathogens. Here, the authors modify and optimize a synthetic genetic system in a probiotic strain of Escherichia coli, and show that the engineered probiotic can sense and kill the pathogen Pseudomonas aeruginosa in two animal models of gut infection.