Polyglycolic acid filaments guide Schwann cell migration in vitro and in vivo
Polyglycolic acid filaments guide Schwann cell migration in vitro and in vivo
复制标题
聚乙醇酸丝在体外和体内指导雪旺细胞迁移
DOI：
10.1007/s10529-008-9795-1
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发表时间：
2008-11-01
影响因子：
2.7
通讯作者：
Gu, Xiaosong
中科院分区：
文献类型：
--
作者：
Hu, Wen;Gu, Jianhui;Gu, Xiaosong
与空心神经导管相比，纵向排列纤维填充的神经导管在桥接大的周围神经间隙方面表现出更好的再生效果。在本研究中，我们采用免疫细胞/组织化学和光学/电子显微镜研究了雪旺细胞在聚乙二醇酸（PGA）细丝上的体外和体外细胞行为。大鼠背根神经节（DRGs）在PGA丝上培养1-3周后，大鼠DRGs中的雪旺细胞粘附在PGA丝上并沿PGA丝迁移。24只大鼠植入经PGA丝植入的壳聚糖导管，以桥接10mm长的坐骨神经间隙。在植入后1、2、3和4周（每个时间点n = 6），发现雪旺细胞沿PGA丝迁移，形成类似Bungner带的细胞柱。这些结果表明PGA纤维可能在雪旺细胞迁移中起接触引导作用，因此可以作为一种有前途的导管填充材料促进周围神经修复。
Nerve conduits filled with longitudinal aligned filaments have demonstrated a better regenerative outcome for bridging large peripheral nerve gaps than hollow nerve conduits. In the present study, we investigated the in vitro and in vitro cellular behavior of Schwann cells on polyglycolic acid (PGA) filaments by immunocyto/histochemistry and light/electron microscopy. After 1-3-week culture of rat dorsal root ganglia (DRGs) onto PGA filaments, Schwann cells from rat DRGs adhered to and migrated along PGA filaments. Twenty-four rats received implantation of chitosan conduits inserted with PGA filaments to bridge 10-mm-long sciatic nerve gaps. At 1, 2, 3 and 4 weeks post-implantation (n = 6, each time point), Schwann cells were found to migrate along PGA filaments and form cell columns resembling bands of Bungner. These results suggest that PGA filaments may play a contact guidance role in Schwann cell migration and thus serve as a promising conduit-filling material to facilitate peripheral nerve repair.