The yolk syncytial layer regulates myocardial migration by influencing extracellular matrix assembly in zebrafish
The yolk syncytial layer regulates myocardial migration by influencing extracellular matrix assembly in zebrafish
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DOI：
10.1242/dev.02581
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发表时间：
2006-10-15
期刊：
影响因子：
4.6
通讯作者：
Stainier, Didier Y. R.
中科院分区：
文献类型：
--
作者：
Sakaguchi, Takuya;Kikuchi, Yutaka;Stainier, Didier Y. R.
胚胎外组织在早期脊椎动物身体模式中的作用已被广泛研究，但我们对它们在后期发育事件中的功能知之甚少。在这里，我们分析了斑马鱼胚胎外卵黄合胞体层（YSL）特异性转录因子Mtx 1的功能，发现它在心肌迁移中起着至关重要的作用。下调YSL中Mtx1的功能会导致心裂，这是一种心肌细胞不能迁移到中线的表型。Mtx1在胚胎外的YSL似乎调节胚胎表达的纤连蛋白，一个基因先前牵连在心肌迁移。我们进一步显示mtx1和纤连蛋白之间的剂量敏感的遗传相互作用。基于这些数据，我们提出，胚胎外YSL调节心肌迁移，至少部分通过影响纤维连接蛋白的表达和随后的组装的细胞外基质在胚胎组织中。
The roles of extra-embryonic tissues in early vertebrate body patterning have been extensively studied, yet we know little about their function during later developmental events. Here, we analyze the function of the zebrafish extra-embryonic yolk syncytial layer (YSL) specific transcription factor, Mtx1, and find that it plays an essential role in myocardial migration. Downregulating the function of Mtx1 in the YSL leads to cardia bifida, a phenotype in which the myocardial cells fail to migrate to the midline. Mtx1 in the extra-embryonic YSL appears to regulate the embryonic expression of fibronectin, a gene previously implicated in myocardial migration. We further show dosage-sensitive genetic interactions between mtx1 and fibronectin. Based on these data, we propose that the extra-embryonic YSL regulates myocardial migration, at least in part by influencing fibronectin expression and subsequent assembly of the extracellular matrix in embryonic tissues.