Alternative trans-splicing of constant and variable exons of a Drosophila axon guidance gene, lola
Alternative trans-splicing of constant and variable exons of a Drosophila axon guidance gene, lola
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果蝇轴突引导基因 lola 的恒定和可变外显子的选择性转拼
DOI：
10.1101/gad.1137303
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发表时间：
2003-10-15
影响因子：
10.5
通讯作者：
Aigaki, T
中科院分区：
文献类型：
--
作者：
Horiuchi, T;Giniger, E;Aigaki, T
lola是一个复杂的果蝇基因，编码至少20种蛋白质亚型，每种亚型都具有相同的N-末端恒定区，连接到不同的C-末端可变区。不同的亚型指定轴突生长和指导的不同方面。我们在这里表明，萝拉mRNA产生的选择性反式剪接外显子顺序编码的相同的DNA链。染色体配对促进等位基因间的反式剪接，允许恒定外显子和可变外显子中的突变之间的互补。我们证明，至少有一个可变的外显子是从自己的启动子转录，并trans-spliced的恒定外显子转录分开。
longitudinals lacking (lola) is a complex Drosophila gene encoding at least 20 protein isoforms, each bearing the same N-terminal constant region linked to a different C-terminal variable region. Different isoforms specify different aspects of axon growth and guidance. We show here that lola mRNAs are generated by alternative trans-splicing of exons sequentially encoded by the same DNA strand. Chromosomal pairing facilitates interallelic trans-splicing, allowing complementation between mutations in the constant and those in the variable exons. We demonstrate that at least one variable exon is transcribed from its own promoter, and trans-spliced to the constant exons transcribed separately.