Age-related base excision repair activity in mouse brain and liver nuclear extracts.
Age-related base excision repair activity in mouse brain and liver nuclear extracts.
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DOI：
10.1093/gerona/58.3.b205
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发表时间：
2003-03
期刊：
影响因子：
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通讯作者：
G. Intano;E. Cho;C. Mcmahan;C. Walter
中科院分区：
文献类型：
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作者：
G. Intano;E. Cho;C. Mcmahan;C. Walter
为了评估相对于年龄的DNA修复活性，使用从不同年龄的小鼠制备的脑和肝核提取物进行体外碱基切除修复测定。与6日龄小鼠相比，在脑核提取物中观察到85%的修复活性下降，在从老年小鼠制备的肝核提取物中观察到50%的修复活性下降。从老年小鼠制备的脑核提取物显示DNA聚合酶-β的丰度降低，但添加纯化蛋白质并没有恢复碱基切除修复活性。其他测试的碱基切除修复蛋白的丰度相对于年龄没有变化。结论是，在衰老过程中，DNA修复的下降可能导致DNA损伤和诱变水平的增加。
To assess DNA repair activity relative to age, in vitro base excision repair assays were performed using brain and liver nuclear extracts prepared from mice of various ages. An 85% decline in repair activity was observed in brain nuclear extracts and a 50% decrease in liver nuclear extracts prepared from old mice compared with 6-day-old mice. Brain nuclear extracts prepared from old mice showed a decreased abundance of DNA polymerase-beta, but the addition of purified protein did not restore base excision repair activity. Abundances of other tested base excision repair proteins did not change relative to age. The conclusion is that, during aging, a decline in DNA repair could contribute to increased levels of DNA damage and mutagenesis.