COMPLETE SEQUENCE OF THE GENOMIC RNA OF ONYONG-NYONG VIRUS AND ITS USE IN THE CONSTRUCTION OF ALPHAVIRUS PHYLOGENETIC TREES
COMPLETE SEQUENCE OF THE GENOMIC RNA OF ONYONG-NYONG VIRUS AND ITS USE IN THE CONSTRUCTION OF ALPHAVIRUS PHYLOGENETIC TREES
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DOI：
10.1016/0042-6822(90)90191-s
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发表时间：
1990-03-01
期刊：
影响因子：
3.7
通讯作者：
STRAUSS, EG
中科院分区：
文献类型：
--
作者：
LEVINSON, RS;STRAUSS, JH;STRAUSS, EG
甲病毒是一组约25种正链RNA病毒，是重要的人类和兽医病原体，在地理上分散。我们在这里报告了甲病毒O '' nyong-nyong病毒基因组RNA的完整核苷酸序列。RNA的长度为11，835个核苷酸，基因组的组织是典型的甲病毒。系统发育树是从O“nyong-nyong和其他六种甲病毒的蛋白质序列构建的。分别为每种非结构和结构病毒蛋白构建树，以检测任何可能的重组事件，以及检查各种蛋白之间的差异分歧。每棵树的成员可以分为三个亚组：塞姆利基森林病毒亚组（塞姆利基森林病毒、奥年尼-尼永病毒和罗斯河病毒）、东部马脑炎病毒亚组（东部马脑炎病毒和委内瑞拉马脑炎病毒）和辛德毕斯病毒亚组。辛德毕斯病毒在地理上仅限于旧世界，与新世界病毒的东部马脑炎亚群的关系比与主要是旧世界病毒的塞姆利基森林病毒亚群的关系更密切。西方马脑炎病毒是一种特殊的情况，因为它是一种重组病毒。它的非结构蛋白和衣壳蛋白与东部马脑炎病毒最接近，而它的糖蛋白与辛德毕斯病毒最接近。一个给定子群的所有成员都从它们的公共结点偏离了相同的量。然而，塞姆利基森林病毒亚组的结构蛋白比东部马脑炎病毒亚组的结构蛋白彼此更密切相关。这种差异可能表明东部马脑炎病毒亚组的成员比塞姆利基森林病毒亚组的成员更早分化，这表明甲病毒起源于新大陆。
The alphaviruses are a group of about 25 positive-strand RNA viruses that are important human and veterinary pathogens and that are geographically dispersed. We report here the complete nucleotide sequence of the genomic RNA of the alphavirus, O''nyong-nyong virus. The RNA is 11,835 nucleotides in length and the organization of the genome is typical of alphaviruses. Phylogenetic trees were constructed from the protein sequences of O''nyong-nyong and six other alphaviruses. Trees were constructed for each nonstructural and structural viral protein individually in order to detect any possible recombinations events, as well as to examine the differential divergence among the various proteins. The members of each tree can be divided into three subgroups: the Semliki Forest virus subgroup (Semliki Forest, O''nyony-nyong, and Ross River viruses), the eastern equine encephalitis virus subgroup (eastern equine encephalitis and Venezuelan equine encephalitis viruses), and the Sindbis virus subgroup. Sindbis virus, which is geographically restricted to the Old World, is more closely related to the eastern equine encephalitis subgroup, which are New World viruses, than it is to the Semliki Forest virus subgroup, which is mostly Old World viruses. Western equine encephalitis virus is a special case because it is a recombinant virus. Its nonstructural and capsid proteins are most closely related to those of eastern equine encephalitis virus while its glycoproteins are most closely related to those of Sindbis virus. All members of a given subgroup have diverged the same amount from their common node point. However, the structural proteins of the Semliki Forest virus subgroup are more closely related to one another than those of the eastern equine encephalitis virus subgroup. This difference probably indicates that the members of the eastern equine encephalitis virus subgroup diverged earlier than the members of the Semliki Forest virus subgroup, which suggests that the alphaviruses originated in the New World.