Insecticidal Activity ofArtemisia vulgarisEssential Oil and Transcriptome Analysis ofTribolium castaneumin Response to Oil Exposure
Insecticidal Activity ofArtemisia vulgarisEssential Oil and Transcriptome Analysis ofTribolium castaneumin Response to Oil Exposure
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蒿精油的杀虫活性和赤拟谷盗对油暴露反应的转录组分析
DOI：
10.3389/fgene.2020.00589
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发表时间：
2020-06-25
影响因子：
3.7
通讯作者：
Li, Bin
中科院分区：
文献类型：
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作者：
Gao, Shanshan;Zhang, Kunpeng;Li, Bin
红粉甲虫（Tribolium castaneum）是世界上最具破坏性的储粮害虫之一。艾草（artemisia vulgaris，艾草）的精油（EO）被认为是一种抑制植物生长、发育和繁殖的强毒性物质。castaneum。然而，a的毒性作用背后的分子机制。vulgarisEO。castaneumremain不清楚。结果表明，暴露于a的红粉甲虫幼虫体内羧酯酶（CarEs）和细胞色素氧化酶P450 （CYPs）两种解毒酶显著升高。vulgarisEO。此外，758个基因在EO处理和对照样品之间存在差异表达。基于基因本体（Gene Ontology， GO）分析，大量差异表达基因（differential expression genes, DEGs）被富集为与生物过程调控、刺激反应、抗原加工和呈递相关的术语。我们的研究结果表明：vulgarisEO干扰了幼虫的抗氧化活性，部分抑制了丝氨酸蛋白酶（SP）、组织蛋白酶（CAT）和脂肪酶信号通路，从而破坏了幼虫的发育和繁殖，下调了应激反应。此外，这些deg表明。黄芪通过诱导铜锌超氧化物歧化酶（CuZnSOD）、血红素过氧化物酶（HPX）、抗氧化酶和转录因子等基因的表达，间接影响甲虫的发育和繁殖。此外，大多数deg在京都基因与基因组百科全书（KEGG）数据库中被映射到药物代谢途径。其中，气味结合蛋白（OBPs） 6个，化学感觉蛋白（CSPs） 5个，cyps 14个，酯酶（ESTs） 3个，谷胱甘肽s -转移酶（GSTs） 5个，udp -葡萄糖醛基转移酶（UGTs） 6个，多药耐药蛋白（MRPs） 2个，其中8CYPs、2ESTs、2GSTs和3ugts在暴露于a后显著上调。vulgarisEO。暴露于EO的幼虫体内，残留DEGs显著下调，表明处理后的甲虫体内存在部分代谢解毒补偿。此外,一个。vulgarisEO诱导obp /CYP过表达，针对这些基因的RNAi显著增加了暴露于EO的幼虫的死亡率，进一步证明obp /CYPin参与EO代谢解毒作用。castaneum。我们的研究结果提供了转录组学变化的概述。castaneumin对a的反应。vulgarisEO。
Red flour beetle (Tribolium castaneum) is one of the most destructive pests of stored cereals worldwide. The essential oil (EO) ofArtemisia vulgaris(mugwort) is known to be a strong toxicant that inhibits the growth, development, and reproduction ofT. castaneum. However, the molecular mechanisms underlying the toxic effects ofA. vulgarisEO onT. castaneumremain unclear. Here, two detoxifying enzymes, carboxylesterase (CarEs) and cytochrome oxidase P450 (CYPs), were dramatically increased in red flour beetle larvae when they were exposed toA. vulgarisEO. Further, 758 genes were differentially expressed between EO treated and control samples. Based on Gene Ontology (GO) analysis, numerous differentially expressed genes (DEGs) were enriched for terms related to the regulation of biological processes, response to stimulus, and antigen processing and presentation. Our results indicated thatA. vulgarisEO disturbed the antioxidant activity in larvae and partially inhibited serine protease (SP), cathepsin (CAT), and lipase signaling pathways, thus disrupting larval development and reproduction as well as down-regulating the stress response. Moreover, these DEGs showed thatA. vulgarisindirectly affected the development and reproduction of beetles by inducing the expression of genes encoding copper-zinc-superoxide dismutase (CuZnSOD), heme peroxidase (HPX), antioxidant enzymes, and transcription factors. Moreover, the majority of DEGs were mapped to the drug metabolism pathway in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Notably, the following genes were detected: 6odorant binding proteins(OBPs), 5chemosensory proteins(CSPs), 14CYPs, 3esterases(ESTs), 5glutathione S-transferases(GSTs), 6UDP-glucuronosyltransferases(UGTs), and 2multidrug resistance proteins(MRPs), of which 8CYPs, 2ESTs, 2GSTs, and 3UGTswere up-regulated dramatically after exposure toA. vulgarisEO. The residual DEGs were significantly down-regulated in EO exposed larvae, implying that partial compensation of metabolism detoxification existed in treated beetles. Furthermore,A. vulgarisEO induced overexpression ofOBP/CYP, and RNAi against these genes significantly increased mortality of larvae exposed to EO, providing further evidence for the involvement ofOBP/CYPin EO metabolic detoxification inT. castaneum. Our results provide an overview of the transcriptomic changes inT. castaneumin response toA. vulgarisEO.