Effects of tetrabromobisphenol A on larval development and thyroid hormone-regulated biomarkers of the amphibian Xenopus laevis
Effects of tetrabromobisphenol A on larval development and thyroid hormone-regulated biomarkers of the amphibian Xenopus laevis
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DOI：
10.1016/j.envres.2005.09.006
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发表时间：
2006-07-01
影响因子：
8.3
通讯作者：
Kloas, Werner
中科院分区：
文献类型：
--
作者：
Jagnytsch, Oana;Opitz, Robert;Kloas, Werner
研究了四溴双酚A （TBBA）对两栖动物非洲爪蟾（Xenopus laevis）甲状腺激素依赖性形态发育和甲状腺激素调节生物标志物的影响。在爪蟾变态实验中，将蝌蚪暴露在2.5 ~ 500 μ g/L不同浓度的TBBA环境中21天。只有最高浓度的TBBA抑制了幼虫的发育，而负反馈机制的生物标志物TH受体β - mrna不受影响。短期暴露于TBBA，三碘甲状腺原氨酸（T3），以及TBBA和T3的组合，以检测th上调的生物标志物的表达。单独使用TBBA可轻微增加TH受体β -和碱性区亮氨酸拉链转录因子b/Zip-mRNA的表达，但以剂量依赖性的方式抑制它们在t3诱导下的升高。尽管TBBA在长期治疗中似乎只对幼虫发育产生轻微影响，但短期暴露显示了间接证据，表明TBBA可以作为TH拮抗剂发挥作用。(c) 2005爱思唯尔公司版权所有。
Effects of tetrabromobisphenol A (TBBA) on thyroid hormone (TH)-dependent morphological development and on TH-regulated biomarkers were investigated using the amphibian Xenopus laevis. Tadpoles were exposed in the Xenopus metamorphosis assay to different TBBA concentrations ranging from 2.5 to 500 mu g/L for 21 days. Only the highest TBBA concentration inhibited larval development while the biomarker for negative feedback mechanism, TH receptor beta-mRNA, was not affected. Short-term exposures to TBBA, triiodothyronine (T3), and a combination of TBBA and T3 were performed to examine expression of TH-upregulated biomarkers. TBBA alone increased slightly the expression of TH receptor beta- and basic region leucin zipper transcription factor b/Zip-mRNA but inhibited their T3-induced elevation in a dose-dependent manner. Despite the fact that TBBA seems to cause only mild effects during long-term treatment on larval development, short-term exposure revealed indirect evidence that TBBA can function as a TH antagonist. (c) 2005 Elsevier Inc. All rights reserved.