In-line monitoring of pH and oxygen during enzymatic reactions in off-the-shelf all-glass microreactors using integrated luminescent microsensors
In-line monitoring of pH and oxygen during enzymatic reactions in off-the-shelf all-glass microreactors using integrated luminescent microsensors
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DOI：
10.1007/s00604-016-2021-2
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发表时间：
2017-02-01
影响因子：
5.7
通讯作者：
Nagl, Stefan
中科院分区：
文献类型：
--
作者：
Pfeiffer, Simon A.;Borisov, Sergey M.;Nagl, Stefan
我们描述了一种无掩模光聚合技术，用于将用于测定pH值和溶解氧（DO）的发光传感器集成到市售的全玻璃微流体反应器中（DO和pH传感器的尺寸均小至43 +/- 3 μ m）。传感器点是聚（乙二醇丙烯酸酯）为基础的，并通过光聚合的低聚物在存在的光学探针的氧气和pH值，分别获得。所得到的设备被施加到在线监测乙酰胆碱和葡萄糖的酶促转化（通过使用乙酰胆碱酯酶和葡萄糖氧化酶，分别）的微型。乙酰胆碱和葡萄糖的检测限分别为258 μ M和505 μ M。该方法具有很大的潜力，在监测酶和其他化学反应的微观尺度上，它不需要特殊的仪器，除了一个标准的宽视场荧光显微镜。
We describe a maskless photopolymerization technique for integration of luminescent sensors for determination of pH values and dissolved oxygen (DO) into commercially available all-glass microfluidic reactors (in sizes as small as 43 +/- 3 mu m for both DO and pH sensors). The sensor spots are poly(ethyleneglycol acrylate)-based and obtained by photopolymerization of oligomers in the presence of optical probes for oxygen and pH values, respectively. The resulting devices were applied to in-line monitoring of enzymatic conversions of acetylcholine and glucose (by using acetylcholine esterase and glucose oxidase, respectively) on the microscale. The detection limits are 258 mu M and 505 mu M for acetylcholine and glucose, respectively. The method has a large potential in terms of monitoring enzymatic and other chemical reactions on the microscale in that it does not require special instrumentation apart from a standard widefield fluorescence microscope.