Ancient olive DNA in pits: preservation, amplification and sequence analysis
Ancient olive DNA in pits: preservation, amplification and sequence analysis
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DOI：
10.1016/j.jas.2005.06.011
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Weiner, S
中科院分区：
文献类型：
--
作者：
Elbaum, R;Melamed-Bessudo, C;Weiner, S
橄榄树（油橄榄）是通过选择野生个体的营养繁殖驯化的，所述野生个体具有上级果实。后来，新的品种建立反复从野生树木或野生，野生和驯化树木之间的杂交。因此，许多当代驯化品系的遗传背景是古代栽培品种和当地野生树木的混合物。古代DNA可能会阐明橄榄驯化的复杂过程，因为此类DNA序列提供了有关更接近驯化事件的古代基因组的数据。保存完好的DNA必须可用于此类研究，即使在地中海地区，橄榄种植发生，气候条件不利于DNA保存。为了选择保存完好的坑，我们通过红外光谱法测量了它们的木质素比例。并将其与DNA质量参数如橄榄特异性重复序列阵列中的模板长度和通过实时PCR扩增确定的模板量相关联。通过这些测试的考古坑确实含有高质量的古代DNA。我们提出了第一个古老的橄榄DNA序列，并将它们与现代野生，野生和驯化的系列进行比较。(c)2005爱思唯尔有限公司保留所有权利。
The olive tree (Olea europaea) was domesticated by vegetative propagation of selected wild individuals with Superior fruit. Later, new cultivars were established repeatedly from feral trees or from crosses between wild, feral, and domesticated trees. Thus the genetic background of many contemporary domesticated lines is a mixture of ancient cultivars and local wild trees. Ancient DNA may illuminate the complicated process of olive domestication because Such DNA sequences provide data about ancient genomes that existed closer to the domestication events. Well preserved DNA must be available for such studies, even though in the Mediterranean region, where olive cultivation took place, the climatic conditions are not favorable for DNA preservation. To select for well preserved pits we measured their proportions of lignin by IR spectroscopy. and correlated this with parameters of DNA quality such as template length in an olive-specific repeat array, and template quantity as determined by real-time PCR amplification. Archaeological pits that passed these tests did contain high quality ancient DNA. We present the first ancient olive DNA sequences and compare them to modern wild, feral and domesticated lines. (c) 2005 Elsevier Ltd. All rights reserved.