THE COCCIDIOIDAL COMPLEMENT-FIXATION AND IMMUNODIFFUSION-COMPLEMENT FIXATION ANTIGEN IS A CHITINASE
THE COCCIDIOIDAL COMPLEMENT-FIXATION AND IMMUNODIFFUSION-COMPLEMENT FIXATION ANTIGEN IS A CHITINASE
复制标题
DOI：
10.1128/iai.60.7.2588-2592.1992
复制
发表时间：
1992-07-01
影响因子：
3.1
通讯作者：
PAPPAGIANIS, D
中科院分区：
文献类型：
--
作者：
JOHNSON, SM;PAPPAGIANIS, D
粗球孢子菌培养物和自溶物为球孢子菌病的血清学诊断和预后判断提供了合适的粗抗原。其中之一，参与对应于补体结合反应（IDCF）的免疫扩散反应的热不稳定抗原，已被表征为110-kDa的天然蛋白质，当经受还原条件和热时，产生48-kDa的组分。本报告提供了血清学和生物化学证据表明，这种抗原是一种几丁质酶。该几丁质酶是从球形内生孢子C相培养48小时的滤液中分离得到的。该抗原与IDCF参考抗原形成一条同源线，并参与与人血清的补体结合反应。当加热时，它失去了其酶活性和抗原活性，但当不加热时，它保留了其酶活性，甚至当与人血清中存在的球孢子抗体沉淀时。这种几丁质酶代表了一种重要的血清学诊断物质，并且可能在C.免疫系统。
Culture filtrates and autolysates of Coccidioides immitis have provided suitable crude antigens for the serodiagnosis and prognosis of coccidioidomycosis. One of these, a heat-labile antigen which participates in the immunodiffusion reaction corresponding to the complement fixation reaction (IDCF), has been characterized as a 110-kDa native protein that, when subjected to reducing conditions and heat, yields a 48-kDa component. The present report provides serologic and biochemical evidence that this antigen is a chitinase. This chitinase, isolated from 48-h culture filtrate of the spherule-endospore-phase C. immitis by affinity adsorption to chitin, formed a line of identity with the IDCF reference antigen and participated in the complement fixation reaction with human serum. It lost its enzymatic as well as antigenic activity when heated, but when not heated it retained its enzymatic activity even when precipitated with coccidioidal antibody present in human serum. This chitinase represents a significant serodiagnostic substance and may be important in the morphogenesis of C. immitis.