HUMAN-MUSCLE GLYCOGEN-SYNTHASE CDNA SEQUENCE - A NEGATIVELY CHARGED PROTEIN WITH AN ASYMMETRIC CHARGE-DISTRIBUTION
HUMAN-MUSCLE GLYCOGEN-SYNTHASE CDNA SEQUENCE - A NEGATIVELY CHARGED PROTEIN WITH AN ASYMMETRIC CHARGE-DISTRIBUTION
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DOI：
10.1073/pnas.86.5.1443
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发表时间：
1989-03-01
影响因子：
11.1
通讯作者：
FLETTERICK, RJ
中科院分区：
文献类型：
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作者：
BROWNER, MF;NAKANO, K;FLETTERICK, RJ
人肌糖原合成酶的cDNA编码737个氨基酸的蛋白质。糖原合成酶的一级结构既与细菌糖原合成酶也与任何糖原磷酸化酶无关。兔肌酶在体内磷酸化的所有9个丝氨酸在人肌序列中都是保守的。含有所有磷酸化位点的氨基端和羧基端片段带很强的负电荷。总的来说，未磷酸化蛋白的电荷为-13，而完全磷酸化的无活性蛋白的净电荷为-31。讨论了电荷不对称分布的重要性。
The cDNA for human muscle glycogen synthase encodes a protein of 737 amino acids. The primary structure of glycogen synthase is not related either to bacterial glycogen synthase or to any glycogen phosphorylase. All nine of the serines that are phosphorylated in the rabbit muscle enzyme in vivo are conserved in the human muscle sequence. The amino- and carboxyl-terminal fragments, which contain all the phosphorylation sites, are very negatively charged. Overall the unphosphorylated protein has a charge of -13, while the fully phosphorylated inactive protein has a net charge of -31. The importance of the asymmetrical charge distribution is discussed.