P-31-NMR STUDY OF SKELETAL-MUSCLE METABOLISM IN PATIENTS WITH CHRONIC RESPIRATORY IMPAIRMENT
P-31-NMR STUDY OF SKELETAL-MUSCLE METABOLISM IN PATIENTS WITH CHRONIC RESPIRATORY IMPAIRMENT
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DOI：
10.1164/ajrccm/146.4.1019
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发表时间：
1992-10-01
期刊：
影响因子：
--
通讯作者：
YAMABAYASHI, H
中科院分区：
文献类型：
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作者：
KUTSUZAWA, T;SHIOYA, S;YAMABAYASHI, H
为了评估慢性呼吸障碍患者运动期间外周骨骼肌的能量代谢，对 9 名患者和 9 名年龄匹配的对照受试者的前臂肌肉 P-31 核磁共振 (NMR) 谱进行了研究。我们计算了磷酸肌酸 (PCr) 与 PCr + 无机磷酸盐 (PI) 的比率、PCr 恢复的时间常数和细胞内 pH 值。该练习包括每 3 秒重复握住 2 公斤负载，持续 6 分钟（0.33 W）。运动期间，患者的 PCr/(PCr + Pi) 比率和肌肉 pH 值显着下降，而对照受试者的 PCr/(PCr + Pi) 显示轻微下降，但 pH 值没有任何变化。 PCr 利用率与 pH 值之间的关系表明，慢性呼吸功能不全患者的无氧糖酵解较早开启。在运动过程中，九名患者中有五名观察到分裂的 Pi 峰值。运动最后一分钟的 PCr/(PCr + Pi) 比率与肺活量（预测百分比）、FEV1/FVC、体重、最大握力以及肌肉质量显着相关。结果表明，慢性呼吸障碍患者的肌肉氧化磷酸化受损，无氧糖酵解早期激活。与慢性呼吸损伤相关的几个因素，例如废用、营养不良和缺氧，会导致在检查的肌肉中观察到的代谢变化。
To evaluate the energy metabolism of peripheral skeletal muscle during exercise in patients with chronic respiratory impairment, the P-31-nuclear magnetic resonance (NMR) spectra of forearm muscle were investigated in nine patients and nine age-matched control subjects. We calculated the phosphocreatine (PCr) to PCr + inorganic phosphate (PI) ratio, the time constant of PCr recovery and the intracellular pH. The exercise consisted of repetitive hand grips against a 2-kg load every 3 s for 6 min (0.33 W). The patients showed a marked decrease in the PCr/(PCr + Pi) ratio and pH in the muscle during exercise in contrast to the control subjects whose PCr/(PCr + Pi) showed a minor decrease without any change in pH. The relationship between PCr utilization and pH demonstrated that anaerobic glycolysis switched on earlier In patients with chronic respiratory impairment. A split Pi peak was observed in five of nine patients during exercise. The PCr/(PCr + Pi) ratio during the last minute of exercise correlated significantly with the vital capacity (% predicted), with the FEV1/FVC, with the body weight, with the maximum strength of hand grip, and with the muscle mass. The results indicate impaired oxidative phosphorylation and the early activation of anaerobic glycolysis in the muscles of patients with chronic respiratory impairment. Several factors related to chronic respiratory impairment, such as disuse, malnutrition, and dysoxia, would contribute to the metabolic changes observed in the muscles examined.