Tissue-specific and developmental pattern of expression of the rice sps1 gene
Tissue-specific and developmental pattern of expression of the rice sps1 gene
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DOI：
10.1104/pp.124.2.641
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发表时间：
2000-10-01
期刊：
影响因子：
7.4
通讯作者：
Herrera-Estrella, L
中科院分区：
文献类型：
--
作者：
Chávez-Bárcenas, AT;Valdez-Alarcón, JJ;Herrera-Estrella, L
蔗糖-磷酸合成酶(SPS)是植物碳同化和分配的关键调节酶之一。SPS在光合作用细胞中产生蔗糖以及在萌发种子中将淀粉或脂肪酸转化为蔗糖方面发挥着核心作用。为探讨SPS的组织特异性和发育分布调控机制，本研究对水稻(Oryza Sativa)sps1(GenBank登录号：U33175)进行原位逆转录聚合酶链式反应，并利用β-葡萄糖苷酸酶报告基因在sps1启动子的引导下进行表达。结果表明，水稻sps1基因仅在叶片叶肉细胞、萌发苗的盾片和未成熟花序的花粉中表达。在叶片发育过程中，sps1启动子引导一种基本的表达模式，这与叶片从库到源的转换过程中SPS活性的分布一致。研究还发现，在生长周期的营养部分，SPS的表达和酶活性在最年轻的充分展开的叶片中最高。此外，还观察到sps1启动子在光合作用组织中的表达受光的调节并依赖于叶绿体的发育，而在盾片中的表达与光和叶绿体的发育无关。
Sucrose-phosphate synthase (SPS) is one of the key regulatory enzymes in carbon assimilation and partitioning in plants. SPS plays a central role in the production of sucrose in photosynthetic cells and in the conversion of starch or fatty acids into sucrose in germinating seeds. To explore the mechanisms that regulate the tissue-specific and developmental distribution of SPS, the expression pattern of rice (Oryza sativa) sps1 (GenBank accession no. U33175) was examined by in situ reverse transcriptase-polymerase chain reaction and the expression directed by the sps1 promoter using the beta -glucuronidase reporter gene. It was found that the expression of the rice sps1 gene is limited to mesophyll cells in leaves, the scutellum of germinating seedlings, and pollen of immature inflorescences. During leaf development, the sps1 promoter directs a basipetal pattern of expression that coincides with the distribution of SPS activity during the leaf sink-to-source transition. It was also found that during the vegetative part of the growth cycle, SPS expression and enzymatic activity are highest in the youngest fully expanded leaf. Additionally, it was observed that the expression of the sps1 promoter is regulated by light and dependent on plastid development in photosynthetic tissues, whereas expression in scutellum is independent of both light and plastid development.