Marinomonas brasilensis sp nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis
Marinomonas brasilensis sp nov., isolated from the coral Mussismilia hispida, and reclassification of Marinomonas basaltis as a later heterotypic synonym of Marinomonas communis
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DOI：
10.1099/ijs.0.024661-0
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发表时间：
2011-05-01
影响因子：
2.8
通讯作者：
Thompson, Fabiano L.
中科院分区：
文献类型：
--
作者：
Chimetto, Luciane A.;Cleenwerck, Ilse;Thompson, Fabiano L.
从位于巴西圣保罗的圣保罗海峡的造礁珊瑚Mussismilia hispida的粘液中分离出一种革兰氏阴性需氧细菌，命名为菌株R-40503（T）。系统发育分析表明，菌株R-40503（T）属于海单胞菌属（MarinomonasL.）R-40503（T）的16 S rRNA基因序列与海洋单胞菌（Marinomonasvaga）、M. basaltis、M. communis和M. pontica，与其他海洋单胞菌属的模式菌株的分离率低于97%。菌株R-40503（T）与遗传学上最接近的海单胞菌属的模式菌株的DNA-DNA杂交（DDH）率低于35%，表明它应被分类为一个新种。扩增片段长度多态性（AFLP），化学分类学和表型分析提供了进一步的证据的建议，一个新的物种。同时，M. basaltis和M.共同体被观察到。这两个种的模式菌株的DDH和AFLP图谱相似性分别为78%和63%。它们的表型特征非常相似，DNA G+C含量相同（46.3mol%）。总的来说，这些数据明确地表明，海洋单胞菌basaltis是一个后来的异型的海洋单胞菌communis的同义词。几种表型特征可用于区分海单胞菌属物种。新菌株R-40503（T）与其邻居明显不同。例如，它显示氧化酶和尿素酶活性，利用L-天冬酰胺并具有脂肪酸C-12：1 3-OH，但缺乏C-10：0和C-12：0。命名为巴西海单胞菌（Marinomonasbrasilensissp. nov.），模式菌株为R-40503（T）（=R-278（T）=LMG 25434（T）=CAIM 1459（T））。菌株R-40503（T）的DNA G+C含量为46.5mol%。
A Gram-negative, aerobic bacterium, designated strain R-40503(T), was isolated from mucus of the reef-builder coral Mussismilia hispida, located in the Sao Sebastiao Channel, Sao Paulo, Brazil. Phylogenetic analyses revealed that strain R-40503(T) belongs to the genus Marinomonas. The 16S rRNA gene sequence similarity of R-40503(T) was above 97% with the type strains of Marinomonas vaga, M. basaltis, M. communis and M. pontica, and below 97% with type strains of the other Marinomonas species. Strain R-40503(T) showed less than 35% DNA-DNA hybridization (DDH) with the type strains of the phylogenetically closest Marinomonas species, demonstrating that it should be classified into a novel species. Amplified fragment length polymorphism (AFLP), chemotaxonomic and phenotypic analyses provided further evidence for the proposal of a novel species. Concurrently, a close genomic relationship between M. basaltis and M. communis was observed. The type strains of these two species showed 78% DDH and 63% AFLP pattern similarity. Their phenotypic features were very similar, and their DNA G+C contents were identical (46.3 mol%). Collectively, these data demonstrate unambiguously that Marinomonas basaltis is a later heterotypic synonym of Marinomonas communis. Several phenotypic features can be used to discriminate between Marinomonas species. The novel strain R-40503(T) is clearly distinguishable from its neighbours. For instance, it shows oxidase and urease activity, utilizes L-asparagine and has the fatty acid C-12:1 3-OH but lacks C-10:0 and C-12:0. The name Marinomonas brasilensis sp. nov. is proposed, with the type strain R-40503(T) (=R-278(T) =LMG 25434(T) =CAIM 1459(T)). The DNA G+C content of strain R-40503(T) is 46.5 mol%.