Identification of an active transposon in intact rice plants
Identification of an active transposon in intact rice plants
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DOI：
10.1007/s00438-005-1131-z
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发表时间：
2005-04-01
影响因子：
3.1
通讯作者：
Kiguchi, T
中科院分区：
文献类型：
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作者：
Fujino, K;Sekiguchi, H;Kiguchi, T
在水稻（Oryza sativa L.）中已经鉴定出在完整植株中有活性的转座因子。607 bp的元件本身，被称为非自主的基于DNA的活性水稻转座子（nDart），没有编码能力。它被发现插入在一个叶绿素缺乏的白化病突变体的基因编码镁原卟啉IX甲基转移酶分离自回交后代来自野生型粳稻品种之间的杂交。nDart具有19-bp末端反向重复序列（TIR），并且在移动时产生8-bp靶位点重复序列（TSD）。在日本晴的基因组序列中鉴定出至少13个nDart元件。数据库搜索确定了较大的元素，称为基于DNA的活性水稻转座子（Dart），其中包含一个ORF的蛋白质，含有一个区域与hAT二聚化基序具有高度相似性。Dart与nDart有几个共同的特征，包括相同的TIR、相似的亚末端序列和产生8-bp的TSD。这些共有的特征表明，非自主元素nDart是自主元素Dart的内部缺失衍生物。我们的结论是，这些活跃的转座子系统属于hAT超家族的II类转座子。由于转座子在完整的水稻植株中是有活性的，它们将成为功能基因组学研究中标记基因的有用工具。
A transposable element that is active in intact plants has been identified in rice (Oryza sativa L.). The 607-bp element itself, termed nonautonomous DNA-based active rice transposon (nDart), has no coding capacity. It was found inserted in the gene encoding Mg-protoporphyrin IX methyltransferase in a chlorophyll-deficient albino mutant isolated from backcross progeny derived from a cross between wild-type japonica varieties. The nDart has 19-bp terminal inverted repeats (TIRs) and, when mobilized, generates an 8-bp target-site duplication (TSD). At least 13 nDart elements were identified in the genome sequence of the japonica cultivar Nipponbare. Database searches identified larger elements, termed DNA-based active rice transposon (Dart) that contained one ORF for a protein that contains a region with high similarity to the hAT dimerization motif. Dart shares several features with nDart, including identical TIRs, similar subterminal sequences and the generation of an 8-bp TSD. These shared features indicate that the nonautonomous element nDart is an internal deletion derivative of the autonomous element Dart. We conclude that these active transposon systems belong to the hAT superfamily of class II transposons. Because the transposons are active in intact rice plants, they should be useful tools for tagging genes in studies of functional genomics.