Human liver glutathione S-transferases: complete primary sequence of an Ha subunit cDNA.
Human liver glutathione S-transferases: complete primary sequence of an Ha subunit cDNA.
复制标题
人肝谷胱甘肽 S-转移酶：Ha 亚基 cDNA 的完整一级序列。
DOI：
10.1016/s0006-291x(86)80358-8
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Qian,B
中科院分区：
文献类型：
--
作者：
Tu,CP;Qian,B
多种具有重叠底物特异性的人肝脏GSH s -转移酶（GST）可能在外源代谢、药物生物转化和抗过氧化损伤保护中发挥多种作用。人肝脏gst由至少两类亚基组成，Ha（Mr=26,000）和Hb（Mr=27,500）。免疫交叉反应和核酸杂交研究表明，人的has亚基与大鼠的Ya、yc亚基及其cdna有密切的关系。我们已经确定了哈亚基1 cDNA的核苷酸序列，pGTH1。其编码序列与大鼠yccdna和yccdna的比对表明，它们的碱基对碱基相同约80%，没有任何缺失或插入。pGTH1与玉米GST cDNA和大鼠GST的Yband yp亚基之间也发现了序列同源区域（bbb50 %）。在大鼠GST超基因家族的62个高度保守的氨基酸残基中，有56个保存在Hasubunit 1编码序列中。比较这些编码序列的氨基酸替换突变，发现大鼠Yaor和Ycgenes之间的差异百分比大于大鼠Yaor和Ycgenes之间的差异百分比。
Multiple human liver GSH S-transferases (GST) with overlapping substrate specificities may be essential to their multiple roles in xenobiotics metabolism, drug biotransformation, and protection against peroxidative damage. Human liver GSTs are composed of at least two classes of subunits, Ha(Mr=26,000) and Hb(Mr=27,500). Immunological cross-reactivity and nucleic acid hybridization studies revealed a close relationship between the human Hasubunit and rat Ya, Ycsubunits and their cDNAs. We have determined the nucleotide sequence of the Hasubunit 1 cDNA, pGTH1. The alignments of its coding sequence with the rat Yaand YccDNAs indicate that they are ∼80% identical base-for-base without any deletion or insertion. Regions of sequence homology (>50%) have also been found between pGTH1 and a corn GST cDNA and rat GST cDNAs of the Yband Ypsubunits. Among the 62 highly conserved amino acid residues of the rat GST supergene family, 56 of them are preserved in the Hasubunit 1 coding sequences. Comparison of amino-acid replacement mutations in these coding sequences revealed that the percentage divergence between the rat Yaand Ycgenes is more than that between the Haand Yaor Haand Ycgenes.