Categorisation of sugar acid dehydratases in Aspergillus niger
Categorisation of sugar acid dehydratases in Aspergillus niger
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DOI：
10.1016/j.fgb.2013.12.006
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发表时间：
2014-03-01
影响因子：
3
通讯作者：
Richard, Peter
中科院分区：
文献类型：
--
作者：
Motter, Francine A.;Kuivanen, Joosu;Richard, Peter
在黑曲霉的基因组中，鉴定出五个基因编码与糖酸脱水酶具有同源性的蛋白质。开放阅读框在酿酒酵母中表达，并用糖酸文库测试活性。鉴定出四个基因编码具有糖酸活性的蛋白质：一种 L-半乳糖酸脱水酶 (gaaB)、两种 D-半乳糖酸脱水酶 (dgdA、dgdB) 和一种 L-鼠李糖酸脱水酶 (lraC)。表征了蛋白质的特异性。 L-半乳糖酸脱水酶对 L-岩藻糖酸具有最高活性，但尚不清楚该酶是否参与 L-岩藻糖酸分解代谢。没有一种蛋白质显示出半乳糖二酸或半乳糖内酯的活性。 (C) 2013 Elsevier Inc. 保留所有权利。
In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an L-galactonate dehydratase (gaaB), two D-galactonate dehydratases (dgdA, dgdB) and an L-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The L-galactonate dehydratase had highest activity with L-fuconate, however it is unclear whether the enzyme is involved in L-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone. (C) 2013 Elsevier Inc. All rights reserved.