Nature of photooxidative events in leaves treated with chlorosis-inducing herbicides.
Nature of photooxidative events in leaves treated with chlorosis-inducing herbicides.
复制标题
用引起失绿的除草剂处理的叶子中光氧化事件的性质。
DOI：
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发表时间：
1982
期刊：
影响因子：
7.4
通讯作者：
Theresia Winkelhüsener
中科院分区：
文献类型：
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作者：
J. Feierabend;Theresia Winkelhüsener
黑麦（Secale cereale L.）在10勒克斯的低光强度下，在四种引起叶绿素变性的除草剂的存在下生长，形成叶绿素。当这种昏暗的光生长的叶片的片段暴露于30，000勒克斯在0摄氏度或30摄氏度，处理与氨基三唑或卤代氧化物（组1）显示没有或只有微小的变化，其叶绿素含量。然而，在处理与San 6706或difunon（组2），叶绿素的快速光破坏发生在0 ℃和30 ℃，并伴随着增加的丙二醛，没有看到在组1除草剂的存在。与在体内的行为，几乎相等的速率的叶绿素分解观察到氨基三唑和San 6706治疗在悬浮液中的分离的叶绿体从10 LUX生长的叶片暴露于强光后。自由基清除剂对苯醌和氢醌和d-青霉胺铜配合物发挥超氧化物歧化活性，有效地防止光氧化的叶绿素在10个生长的除草剂处理的叶段，甚至恢复叶绿素在30,000勒克斯的积累。抗坏血酸和几种单线态氧或羟基自由基清除剂没有保护作用。氧化氘和H2 O2对叶绿素的降解没有促进作用。超氧化物歧化酶活性下降，在第2组除草剂的存在下漂白的叶片。
Leaves of rye seedlings (Secale cereale L.) grown in the presence of four chlorosis-inducing herbicides under a low light intensity of 10 lux formed chlorophyll. When segments of such dim-light-grown leaves were exposed to 30,000 lux at either 0 degrees C or 30 degrees C, treatments with aminotriazole or haloxidine (group 1) showed no or only minor changes of their chlorophyll contents. In treatments with San 6706 or difunon (group 2), however, rapid photodestruction of chlorophyll occurred both at 0 degrees C and at 30 degrees C and was accompanied by an increase of malondialdehyde that was not seen in the presence of group 1 herbicides. Unlike the in vivo behavior, virtually equal rates of chlorophyll breakdown were observed for aminotriazole and San 6706 treatments in suspensions of isolated chloroplasts from 10 lux-grown leaves after exposure to strong light. The free radical scavengers p-benzoquinone and hydroquinone and the d-penicillamine copper complex exerting superoxide dismutating activity effectively prevented photooxidation of chlorophyll in 10 lux-grown herbicide-treated leaf segments or even restored an accumulation of chlorophyll at 30,000 lux. Ascorbate and several singlet oxygen or hydroxyl radical scavengers had no protective effects. Deuterium oxide and H(2)O(2) did not enhance the degradation of chlorophyll. Superoxide dismutase activity was decreased in leaves bleached in the presence of group 2 herbicides.