2 DISTINCT GENE SUBFAMILIES WITHIN THE FAMILY OF CYSTEINE PROTEASE GENES
2 DISTINCT GENE SUBFAMILIES WITHIN THE FAMILY OF CYSTEINE PROTEASE GENES
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DOI：
10.1073/pnas.90.7.3063
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发表时间：
1993-04-01
影响因子：
11.1
通讯作者：
DITOMAS, ME
中科院分区：
文献类型：
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作者：
KARRER, KM;PEIFFER, SL;DITOMAS, ME
对生理调节的四膜虫半胱氨酸蛋白酶基因的cDNA克隆进行测序。核苷酸序列预测该克隆编码由19个残基的N-末端信号序列、随后的107个残基的前肽和210个残基的成熟蛋白组成的336个氨基酸的蛋白。与其他半胱氨酸蛋白酶的蛋白质的推导的氨基酸序列的比较，揭示了一个高度保守的散布在蛋白质的前肽区域，ERFNIN基序的氨基酸基序。该基序存在于数据库中的所有半胱氨酸蛋白酶中，除了组织蛋白酶B样蛋白，其具有较短的前肽。成熟蛋白的前肽和保守氨基酸的差异表明ERFNIN蛋白酶和组织蛋白酶B样蛋白酶构成半胱氨酸蛋白酶内的两个不同的亚家族。
A cDNA clone for a physiologically regulated Tetrahymena cysteine protease gene was sequenced. The nucleotide sequence predicts that the clone encodes a 336-amino acid protein composed of a 19-residue N-terminal signal sequence followed by a 107-residue propeptide and a 210-residue mature protein. Comparison of the deduced amino acid sequence of the protein with those of other cysteine proteases revealed a highly conserved interspersed amino acid motif in the propeptide region of the protein, the ERFNIN motif. The motif was present in all of the cysteine proteases in the data base with the exception of the cathepsin B-like proteins, which have shorter propeptides. Differences in the propeptides and in conserved amino acids of the mature proteins suggest that the ERFNIN proteases and the cathepsin B-like proteases constitute two distinct subfamilies within the cysteine proteases.