Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA
Efficient transformation of filamentous fungus Pleurotus ostreatus using single-strand carrier DNA
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DOI：
10.1007/s002530000535
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发表时间：
2001-05-01
影响因子：
5
通讯作者：
Kuwahara, M
中科院分区：
文献类型：
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作者：
Irie, T;Honda, Y;Kuwahara, M
分析了载体dna对担子菌平菇转化的影响。将λ噬菌体DNA加入到含有原生质体和Cbx(R)载体质粒的转化混合物中，在含有2杯carboxin/ml的筛选板上观察到耐药转化体数量增加。通过添加热变性lambda DNA获得了最高的效率（约200个转化子/杯载体质粒），其产量比不添加载体DNA的对照实验高约50倍。据我们所知，这是关于单链载体DNA提高真菌原生质体转化效率的第一篇报道。
The effects of carrier DNAs on the transformation of the basidiomycete Pleurotus ostreatus were analyzed. When lambda phage DNA was added to a transformation mixture containing protoplasts and Cbx(R) vector plasmid, an increased number of drug-resistant transformants was observed on a screening plate containing 2 mug carboxin/ml. The highest efficiency (about 200 transformants/mug Vector plasmid) was obtained by the addition of heat-denatured lambda DNA, which gave yields approximately 50-fold higher than the control experiment without a carrier DNA. To our knowledge, this is the first report on enhancement in transformation efficiency of fungal protoplasts by single strand carrier DNA.