Algal biofuel: bountiful lipid from Chlorococcum sp. proliferating in municipal wastewater
Algal biofuel: bountiful lipid from Chlorococcum sp. proliferating in municipal wastewater
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藻类生物燃料：来自绿球藻的丰富脂质。
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
T. Ramachandra
中科院分区：
文献类型：
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作者：
D. Mahapatra;T. Ramachandra
藻类生物燃料已成为可行的可再生能源，并且是近年来化石燃料的潜在替代品。除了处理废水外，藻类还有潜力产生大量商业上可行的生物燃料。三种藻类，即。绿球藻属、微囊藻属。和席藻属 sp。在实验室中，在类似的废水条件下，分离并培养了在废水池中增殖的细菌。绿球藻属平均生物量生产力为0.09克。 I(-1)d(-1)在培养实验的第九天具有最大`生物量密度1.33g I-1和相对较高的脂质含量30.55％(w/w)。在类似条件下，微囊藻属 sp。和席藻属 sp。平均生物量生产率分别为0.058和0.063 g I-1 d(-1)，总脂质含量分别为8.88%和18.66%。通过比较碳水化合物与蛋白质的比率来进行生化成分（碳水化合物、蛋白质、脂质和磷酸盐）变化和脂质积累研究；使用衰减全反射傅里叶变换红外光谱分析脂质与蛋白质 (L/P) 和脂质与磷酸盐的关系，结果显示在藻类生长的稳定阶段具有较高的 L/P 比。使用气相色谱法和质谱法对脂肪酸甲酯进行了成分分析。绿球藻属具有更高的生产力和更快的生长速度，具有更高的脂质含量，约 67% 的饱和脂肪酸以棕榈酸酯 (36.3%) 为主，其次是亚油酸酯 (14%) 等不饱和脂肪酸，与其他废水种植物种相比，已被证明是一种经济且可行的生物燃料生产原料。
Algae biofuel have emerged as viable renewable energy sources and are the potential alternatives to fossil-based fuels in recent times. Algae have the potential to generate significant quantities of commercially viable biofuel apart from treating wastewater. Three algal species, viz. Chlorococcum sp., Microcystis sp. and Phormidium sp. proliferating in wastewater ponds were isolated and cultured in the laboratory myxotrophically under similar wastewater conditions. Chlorococcum sp. attained a mean biomass productivity of 0.09 g. I(-1)d(-1) with the maximum `biomass density of 1.33 g I-1 and comparatively higher lipid content of 30.55% (w/w) on the ninth day of the culture experiment. Under similar conditions Microcystis sp. and Phormidium sp. attained mean biomass productivities of 0.058 and 0.063 g I-1 d(-1) with a total lipid content of 8.88% and 18.66% respectively. Biochemical composition (carbohydrates, proteins, lipids and phosphates) variations and lipid accumulation studies were performed by comparison of the ratios of carbohydrate to protein; lipid to protein (L/P) and lipid to phosphates using attenuated total reflectance-Fourier transform infrared spectroscopy which showed higher L/P ratio during the stationary phase of algal growth. Composition analysis of fatty acid methyl ester has been performed using gas chromatography and mass spectrometry. Chlorococcum sp. with higher productivity and faster growth rate has higher lipid content with about 67% of saturated fatty acid dominated by palmitate (36.3%) followed by an unsaturate as linoleate (14%) and has proved to be an economical and viable feedstock for biofuel production compared to the other wastewater-grown species.