Effect of Additives on the Selectivity and Reactivity of Enzymes
Effect of Additives on the Selectivity and Reactivity of Enzymes
复制标题
添加剂对酶选择性和反应性的影响
DOI：
10.1002/tcr.201600016
复制
发表时间：
2017
期刊：
影响因子：
6.6
通讯作者：
Lin Xian-Fu
中科院分区：
文献类型：
--
作者：
Liang Yi-Ru;Wu Qi;Lin Xian-Fu
酶因其反应条件温和、选择性和效率高而被广泛用作有机化学中高效、环保、可生物降解的催化剂。近年来，许多酶在非自然反应中的催化混杂性被化学家和生物化学家揭示和研究，扩大了酶的应用潜力。为了增强酶在自然或混杂反应中的选择性和活性，已经推荐了许多方法，例如蛋白质工程、过程工程和介质工程。其中，加法方法因其使用简单且效率高而非常有吸引力。在本文中，我们将回顾添加剂在提高酶在自然反应和混杂反应中的催化性能方面的应用的最新进展。这些添加剂包括水、有机碱、水模拟物、助溶剂、冠醚、盐、表面活性剂和一些特定的分子添加剂。
Enzymes have been widely used as efficient, eco‐friendly, and biodegradable catalysts in organic chemistry due to their mild reaction conditions and high selectivity and efficiency. In recent years, the catalytic promiscuity of many enzymes in unnatural reactions has been revealed and studied by chemists and biochemists, which has expanded the application potential of enzymes. To enhance the selectivity and activity of enzymes in their natural or promiscuous reactions, many methods have been recommended, such as protein engineering, process engineering, and media engineering. Among them, the additive approach is very attractive because of its simplicity to use and high efficiency. In this paper, we will review the recent developments about the applications of additives to improve the catalytic performances of enzymes in their natural and promiscuous reactions. These additives include water, organic bases, water mimics, cosolvents, crown ethers, salts, surfactants, and some particular molecular additives.