Survey of resistance gene analogs in Solanum caripense, a relative of potato and tomato, and update on R gene genealogy
Survey of resistance gene analogs in Solanum caripense, a relative of potato and tomato, and update on R gene genealogy
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DOI：
10.1007/s00438-005-0038-z
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发表时间：
2005-12-01
影响因子：
3.1
通讯作者：
Trognitz, BR
中科院分区：
文献类型：
--
作者：
Trognitz, FC;Trognitz, BR
TIR-和非TIR（或CC-）超家族的抗性（R）蛋白具有核苷酸结合位点（NBS）结构域。在R基因中，NBS是最高保守性的区域，这表明在触发R蛋白活性中起重要作用。我们比较了从S.利用特异性简并引物，通过PCR方法从其它植物中扩增出Caripense基因组DNA。一个整体高度的序列保守性是明显的整个P-环，激酶-2和激酶-3a基序的NBS片段从所有植物。在R基因的非TIR类中，检测到与赋予晚疫病抗性的马铃薯R1基因相似的突出的子类。所有测序的非TIR-R1样R基因片段具有完整的开放阅读框，而22%的非TIR-非R1样片段和59%的TIR-NBS RGA片段具有中断的阅读框或含有转座子特异性序列。非TIR-R1类片段与茄科植物的R基因相似性较高，与其他植物的RGA相似性较低。和谷物。它的结论是，非TIR-R1样NBS域的外观代表了一个相对较新的进化发展。
The resistance (R) proteins of the TIR- and non-TIR (or CC-) superfamilies possess a nucleotide binding site (NBS) domain. Within an R gene, the NBS is the region of highest conservation, suggesting an essential role in triggering R protein activity. We compared the NBS domain of functional R genes and resistance gene analogs (RGA) amplified from S. caripense genomic DNA via PCR using specific and degenerate primers with its counterpart from other plants. An overall high degree of sequence conservation was apparent throughout the P-loop, kinase-2 and kinase-3a motifs of NBS fragments from all plants. Within the non-TIR class of R genes a prominent sub-class similar to the potato R1 gene conferring resistance to late blight, was detected. All non-TIR-R1-like R gene fragments that were sequenced possessed an intact open reading frame, whereas 22% of all non-TIR-non-R1-like fragments and 59% of all TIR-NBS RGA fragments had an interrupted reading frame or contained transposon-specific sequence. The non-TIR-R1-like fragments had high similarity to Solanaceae R genes and low similarity to RGAs of other plant species including A. thaliana and the cereals. It is concluded that appearance of the non-TIR-R1-like NBS domain represents a relatively recent evolutionary development.