ANATOMY OF HERPES-SIMPLEX VIRUS-DNA .12. ACCUMULATION OF HEAD-TO-TAIL CONCATEMERS IN NUCLEI OF INFECTED CELL AND THEIR ROLE IN THE GENERATION OF THE 4 ISOMERIC ARRANGEMENTS OF VIRAL-DNA
ANATOMY OF HERPES-SIMPLEX VIRUS-DNA .12. ACCUMULATION OF HEAD-TO-TAIL CONCATEMERS IN NUCLEI OF INFECTED CELL AND THEIR ROLE IN THE GENERATION OF THE 4 ISOMERIC ARRANGEMENTS OF VIRAL-DNA
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DOI：
10.1128/jvi.29.2.448-457.1979
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发表时间：
1979-01-01
影响因子：
5.4
通讯作者：
ROIZMAN, B
中科院分区：
文献类型：
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作者：
JACOB, RJ;MORSE, LS;ROIZMAN, B
单纯疱疹病毒DNA是由4组线性分子组成的，其中大组分(L组分)和小组分(S组分)的共价连接方向不同。病毒DNA的这4种异构体排列一起显示了4种不同的末端和4种不同的L-S成分连接。用限制性内切酶[BglII、BglIII、Hsui、EcoRI和HPAI]分析新复制的病毒DNA在病毒DNA合成开始后不久，后代DNA在感染后期积聚在细胞核中，并在感染后8h迅速沉积在细胞核中。在每一种情况下，核病毒DNA中所有4个末端片段的浓度都降低，跨越L和S组分交界处的片段相对于其他DNA片段的浓度增加。因此，积累在细胞核中的病毒DNA由头到尾的串联体组成，这些串联体是通过滚环机制复制DNA而产生的。基于单位长度DNA从头到尾串联链的切除和修复，以及病毒DNA序列排列的已知特征，提出了一个从1种排列产生全部4种单纯疱疹病毒DNA异构体的模型。
Herpes simplex virus DNA extracted from virions accumulating in the cytoplasm of infected cells consists of 4 populations of linear molecules differing in the orientation of the covalently linked large (L) and small (S) components relative to each other. Together, these 4 isomeric arrangements of viral DNA display 4 different termini and 4 different L-S component junctions. Restriction endonucleases [BglII, BglIII, HsuI, EcoRI and HpaI] were used to analyze the newly replicated viral DNA shortly after the onset of viral DNA synthesis, the progeny DNA accumulating in the nuclei late in infection and rapidly sedimenting DNA present in nuclei of infected [African green monkey kidney Vero] cells at 8 h after infection. In each instance the nuclear viral DNA contained a decreased concentration of all 4 terminal fragments and an increase in the concentration of fragments spanning the junction of L and S components relative to the concentration of other DNA fragments. Thus the viral DNA accumulating in the nuclei consists of head-to-tail concatemers arising from the replication of DNA by a rolling-circle mechanism. A model is presented for generation of all 4 isomeric arrangements of herpes simplex virus DNA from 1 arrangement based on excision and repair of unit length DNA from head-to-tail concatemers and known features of the sequence arrangement of viral DNA.