Streaming instability in growing cell populations.
Streaming instability in growing cell populations.
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DOI：
10.1103/physrevlett.104.208101
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发表时间：
2010-05-21
影响因子：
8.6
通讯作者：
Tsimring LS
中科院分区：
文献类型：
--
作者：
Mather W;Mondragón-Palomino O;Danino T;Hasty J;Tsimring LS
在有限空间中生长为准单层的细胞流可以表现出流动，快速移动的细胞的窄流围绕缓慢移动的细胞簇流动。实验观察并分析了E.大肠杆菌细菌增殖的微流控细胞陷阱使用延时显微镜。我们还进行了连续模拟和离散元模拟来阐明流动不稳定性背后的机制。我们的分析表明，流可以解释为细胞的缓慢适应其局部微环境和其流动性之间的相互作用，由于细胞-基底接触力的变化。
Flows of cells growing as a quasimonolayer in a confined space can exhibit streaming, with narrow streams of fast-moving cells flowing around clusters of slowly moving cells. We observed and analyzed this phenomenon experimentally for E. coli bacteria proliferating in a microfluidic cell trap using time-lapse microscopy. We also performed continuum modeling and discrete-element simulations to elucidate the mechanism behind the streaming instability. Our analysis demonstrates that streaming can be explained by the interplay between the slow adaptation of a cell to its local microenvironment and its mobility due to changes of cell-substrate contact forces.