A seasonal study of the mecA gene and Staphylococcus aureus including methicillin-resistant S. aureus in a municipal wastewater treatment plant
A seasonal study of the mecA gene and Staphylococcus aureus including methicillin-resistant S. aureus in a municipal wastewater treatment plant
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DOI：
10.1016/j.watres.2008.11.036
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发表时间：
2009-03-01
期刊：
影响因子：
12.8
通讯作者：
Lindgren, Per-Eric
中科院分区：
文献类型：
--
作者：
Borjesson, Stefan;Melin, Sara;Lindgren, Per-Eric
mecA 基因介导耐药性的耐甲氧西林金黄色葡萄球菌 (MRSA) 的传播威胁着葡萄球菌疾病的治疗。目的是确定废水处理过程对 mecA 基因浓度以及金黄色葡萄球菌和 MRSA 随时间推移的流行情况的影响。为了实现这一目标，使用实时 PCR 检测对市政污水处理厂的 mecA 基因、金黄色葡萄球菌和 MRSA 进行了调查。一年内每月在工厂的八个地点采集水样，反映处理过程的不同方面。所有采样点全年均可检测到 mecA 基因和金黄色葡萄球菌。 MRSA 也可以被检测到，但主要是在早期治疗步骤中。通过进水培养证实了 MRSA 的存在。 mecA 基因的浓度随月份和采样地点的不同而变化，但无法确定明显的季节性变化。废水处理过程在大多数月份降低了mecA基因浓度。总而言之，我们的结果表明，mecA 基因、金黄色葡萄球菌和 MRSA 在一年中出现在所有调查地点。 (C) 2008 Elsevier Ltd. 保留所有权利。
The spread of methicillin-resistant Staphylococcus aureus (MRSA), in which the mecA gene mediates resistance, threatens the treatment of staphylococcal diseases. The aims were to determine the effect of wastewater treatment processes on mecA gene concentrations, and the prevalence of S. aureus and MRSA over time. To achieve this a municipal wastewater treatment plant was investigated for the mecA gene, S. aureus and MRSA, using real-time PCR assays. Water samples were collected monthly for one year, at eight sites in the plant, reflecting different aspects of the treatment process. The mecA gene and S. aureus could be detected throughout the year at all sampling sites. MRSA could also be detected, but mainly in the early treatment steps. The presence of MRSA was verified through cultivation from inlet water. The concentration of the mecA gene varied between months and sampling sites, but no obvious seasonal variation could be determined. The wastewater treatment process reduced the mecA gene concentration in most months. Taken together our results show that the mecA gene, S. aureus and MRSA occur over the year at all sites investigated. (C) 2008 Elsevier Ltd. All rights reserved.