Morphological and secretory properties of bronchoalveolar lavage mast cells in respiratory diseases
Morphological and secretory properties of bronchoalveolar lavage mast cells in respiratory diseases
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呼吸系统疾病中支气管肺泡灌洗肥大细胞的形态和分泌特性
DOI：
10.1111/j.1365-2222.1986.tb00760.x
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发表时间：
1986
影响因子：
6.1
通讯作者：
A. B. Kay
中科院分区：
文献类型：
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作者：
A. Wardlaw;O. Cromwell;D. Celestino;P. Fitzharris;D. M. Geddes;J. V. Collins;A. B. Kay
We have studied various functional and morphological characteristics of mast cells obtained in bronchoalveolar lavage from fifty‐two patients with several lung diseases. The percentage of mast cells ranged from 0.04 to 0.6% (bronchial carcinoma). 0.05–0.3% (sarcoidosis), 0.06–0.25% (asthma), 0.04–1.8% (miscellaneous) and 0.02–0.04% (normals). There were no significant differences in the mast cell counts between the disease groups. Lung mast cells exhibited heterogeneity of size, shape and intensity of staining. Cells from thirty‐seven subjects were further studied for total histamine content and histamine release using various secretagogues. There was a significant correlation (P<0.001) between the histamine content of the total lavage cell population and mast cell counts. The calculated mean histamine content per mast cell was 6.35 pg. Histamine was released in a dose‐dependent fashion after stimulation with anti‐IgE, calcium ionophore and phorbol myristate acetate with a time course of histamine release characteristic of the mast cell. Unlike peripheral blood basophils, no release was observed following incubation with f‐met‐leu‐phe (10−6‐10−8m) and neither cell type released histamine following incubation with 48/80 (10 μg/ml). Inhibition of anti‐IgE‐induced histamine release was obtained following pre‐incubation with salbutamol (10−4‐10−6m). These studies indicate that bronchoalveolar lavage is a suitable model for the study of human lung mast cells.
抗炎类固醇抑制嗜碱性粒细胞组胺的释放。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Schleimer,RP;MacGlashanJr,DW;Gillespie,E;Lichtenstein,LM
通讯作者：
Lichtenstein,LM