WDR13 promotes the differentiation of bovine skeletal muscle-derived satellite cells by affecting PI3K/AKT signaling
WDR13 promotes the differentiation of bovine skeletal muscle-derived satellite cells by affecting PI3K/AKT signaling
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WDR13 通过影响 PI3K/AKT 信号传导促进牛骨骼肌衍生卫星细胞的分化
DOI：
10.1002/cbin.11160
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发表时间：
2019-07-01
影响因子：
3.9
通讯作者：
Yan, Yunqin
中科院分区：
文献类型：
--
作者：
Fu, Yuying;Li, Shuang;Yan, Yunqin
肌肉卫星细胞通常处于静止状态，当外界刺激或调节时，它们可以通过细胞融合进一步分化，形成新的肌管和肌纤维。WD重复结构域13(WDR13)在脊椎动物中高度保守。研究表明，缺乏Wdr13基因的小鼠会出现轻度肥胖、高胰岛素血症和胰岛β细胞增殖增加。然而，WDR13在牛细胞中的作用尚不清楚。在这里，我们研究了WDR13对牛骨骼肌源性卫星细胞(MDSCs)的影响。我们通过Western blotting和免疫荧光实验发现WDR13在牛MDSCs中表达上调。此外，WDR13表达的激活和抑制分别增加和减少了细胞分化，提示WDR13促进了牛MDSC的分化。为了进一步了解WDR13的作用机制，我们研究了WDR13激活或抑制后PI3K/AKT信号通路的变化。此外，用磷脂酰肌醇激酶3(PI3K)抑制剂LY294004处理细胞以观察细胞分化。结果表明，WDR13的激活抑制了PI3K/AKT信号通路，促进了细胞分化。提示WDR13可能通过影响PI3K/AKT信号通路促进牛MDSCs的分化。
Muscle satellite cells are usually at rest, and when externally stimulated or regulated, they can be further differentiated by cell fusion to form new myotubes and muscle fibers. WD repeat domain 13 (WDR13) is highly conserved in vertebrates. Studies have shown that mice lacking the Wdr13 gene develop mild obesity, hyperinsulinemia, and increased islet beta cell proliferation. However, the role of WDR13 in bovine cells is unclear. Here, we investigated the effect of WDR13 on bovine skeletal muscle-derived satellite cells (MDSCs). We found that WDR13 was upregulated in bovine MDSCs using western blotting and immunofluorescence experiments. Moreover, activation and inhibition of WDR13 expression increased and decreased cell differentiation, respectively, suggesting that WDR13 promotes bovine MDSC differentiation. To further understand the mechanism of action of WDR13, we examined changes in the PI3K/AKT signaling pathway following WDR13 activation or inhibition. In addition, cells were treated with a phosphoinositide kinase 3 (PI3K) inhibitor, LY294004, to observe cell differentiation. The results showed that activation of WDR13 inhibited the PI3K/AKT signaling pathway and enhanced cell differentiation. These data suggest that WDR13 can promote the differentiation of bovine MDSCs by affecting the PI3K/AKT signaling pathway.