Attachment of potential cultivable primo-colonizing bacteria and its implications on the fate of low-density polyethylene (LDPE) plastics in the marine environment.
Attachment of potential cultivable primo-colonizing bacteria and its implications on the fate of low-density polyethylene (LDPE) plastics in the marine environment.
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DOI：
10.1016/j.jhazmat.2023.131124
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发表时间：
2023-03
影响因子：
13.6
通讯作者：
Justine Marey S. Bitalac;N. Lantican;N. C. Gomez;D. F. Onda
中科院分区：
文献类型：
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作者：
Justine Marey S. Bitalac;N. Lantican;N. C. Gomez;D. F. Onda
释放到环境中的塑料成为微生物附着和定植的合适基质。与塑料相关的微生物群落相互作用，并且在代谢上与周围环境不同。然而，先锋殖民物种和他们的相互作用与塑料在最初的殖民较少的描述。以低密度聚乙烯（LDPE）为唯一碳源，采用双重选择性富集法，从马尼拉湾的海洋沉积物中分离得到细菌。根据16 S rRNA基因的同源性，将10个菌株鉴定为盐单胞菌属（Halomonas）、芽孢杆菌属（Bacillus）、交替单胞菌属（Alteromonas）、发光杆菌属（Photobacterium）和阿利什米氏菌属（Aliishimia），其中大部分菌株具有表面相关的生活方式。然后通过与LDPE片材共孵育60天，测试分离株在聚乙烯（PE）上定殖的能力。裂缝中菌落的生长、细胞状凹坑的形成以及表面粗糙度的增加表明物理劣化。傅里叶变换红外（FT-IR）光谱显示显着的变化，分别与分离物共孵育的LDPE片材上的官能团和键指数，表明不同的物种可能针对不同的基板的光氧化的聚合物骨架。了解塑料表面上的原生定殖细菌的活动可以提供有关使塑料对其他物种更具生物利用度的可能机制的见解，以及它们对塑料在海洋环境中的命运的影响。
Plastics released in the environment become suitable matrices for microbial attachment and colonization. Plastics-associated microbial communities interact with each other and are metabolically distinct from the surrounding environment. However, pioneer colonizing species and their interaction with the plastic during initial colonization are less described. Marine sediment bacteria from sites in Manila Bay were isolatedviaa double selective enrichment method using sterilized low-density polyethylene (LDPE) sheets as the sole carbon source. Ten isolates were identified to belong to the generaHalomonas,Bacillus,Alteromonas,Photobacterium, andAliishimiabased on 16S rRNA gene phylogeny, and majority of the taxa found exhibit a surface-associated lifestyle. Isolates were then tested for their ability to colonize polyethylene (PE) through co-incubation with LDPE sheets for 60 days. Growth of colonies in crevices, formation of cell-shaped pits, and increased roughness of the surface indicate physical deterioration. Fourier-transform infrared (FT-IR) spectroscopy revealed significant changes in the functional groups and bond indices on LDPE sheets separately co-incubated with the isolates, demonstrating that different species potentially target different substrates of the photo-oxidized polymer backbone. Understanding the activity of primo-colonizing bacteria on the plastic surface can provide insights on the possible mechanisms used to make plastic more bioavailable for other species, and their implications on the fate of plastics in the marine environment.