Retinal dehydrogenase gene expression in stomach and small intestine of rats during postnatal development and in vitamin A deficiency
Retinal dehydrogenase gene expression in stomach and small intestine of rats during postnatal development and in vitamin A deficiency
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DOI：
10.1016/s0014-5793(98)00355-x
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发表时间：
1998-04-17
期刊：
影响因子：
3.5
通讯作者：
Bhat, PV
中科院分区：
文献类型：
--
作者：
Bhat, PV
视网膜脱氢酶（RALDH）催化将视网膜氧化为全反式和9-顺式视黄酸，其作为控制RXR和RXR核受体信号传导途径的配体起作用。我们最近通过逆转录聚合酶链反应显示RALDH转录物在胃和小肠中的表达[Bhat，P. V.，拉布雷克，大仲马，F.，Lacrois，A.和Yoshida，A.（1995）Gene 166，303-306]，我们通过测定mRNA水平和蛋白质以及酶活性来检测出生前和出生后发育期间以及维生素A缺乏时胃和小肠中的RALDH表达。在~ 2天的胎儿中，RALDH在小肠中表达高，而RALDH蛋白在胃中检测不到。RALDH的表达从出生后开始在胃中出现，随日龄逐渐增加，42日龄时达到最高水平。在肠道中，出生后RALDH表达减少。维生素A缺乏上调RALDH在胃和小肠的表达，和管理维甲酸下调RALDH在这些组织中的表达。这些结果表明RALDH在出生后发育过程中在胃和小肠中的差异表达，并且维生素ri状态调节RALDH基因在这些组织中的表达。(C)1998年欧洲生物化学学会联合会。
Retinal dehydrogenase (RALDH) catalyzes the oxidation of retinal to all-trans and 9-cis retinoic acid, which function as ligands controlling EAR and RXR nuclear receptor-signaling pathways. We have recently shown the expression of RALDH transcript in the stomach and small intestine by reverse transcription polymerase chain reaction [Bhat, P.V., Labrecque J., Dumas, F., Lacrois, A. and Yoshida, A. (1995) Gene 166, 303-306], We have examined RALDH expression in the stomach and small intestine before and during postnatal development and in vitamin A deficiency by assaying for mRNA levels and protein as well as for enzyme activity, In -2 day fetuses, RALDH expression was high in the small intestine, whereas RALDH protein was not detectable in the stomach, However, expression of RALDH was seen in the stomach after birth, and gradually increased dth age and reached the highest level at postnatal day 42. In the intestine, RALDH expression decreased postnatally. Vitamin A deficiency up-regulated RALDH expression in the stomach and small intestine, and administration of retinoids down-regulated the RALDH expression in these tissues. These results show the differential expression of RALDH in the stomach and small intestine during postnatal development, and that vitamin ri status regulates the expression of RALDH gene in these tissues. (C) 1998 Federation of European Biochemical Societies.